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Visar resultat 1 - 5 av 10 avhandlingar som matchar ovanstående sökkriterier.

1. 1. DNA Electrophoresis in Polymer Solutions. Separation Mechanisms studied by Fluorescence and Linear Dichroism Spectroscopy

   Författare :Christina Carlsson; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; mutation detection; orientation; YOYO; DNA electrophoresis; polarized light spectroscopy; optical probing; polymer solutions; fluorescence microscopy; PNA; binding modes;

   Sammanfattning : Capillary electrophoresis of DNA in polymer solutions is a promising technique for separation of DNA molecules according to size. The primary aim of the work presented in this thesis was to get a better understanding of the electrophoretic separation mechanism in these media by studying the DNA migration behavior during electrophoresis in both entangled and non-entangled polymer solutions using linear dichroism spectroscopy and fluorescence video microscopy. LÄS MER

3. 2. DNA Electrophoresis: Optical Probing and Microscopic Imaging to Understand Separation Mechanisms

   Författare :Anette Larsson; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; optical probing; YOYO; polarized light spectroscopy; DNA electrophoresis; DAPI; binding modes; helix alignment; fluorescence microscopy;

   Sammanfattning : The aim of this work was to combine spectroscopic, mobility, and fluorescence microscopy studies to elucidate the mechanisms behind DNA electrophoresis, a method to separate DNA molecules according to size. The microscopy studies require staining of the DNA with a fluorescent dye. LÄS MER

4. 3. Amyloid Fibril Formation - Dye Detection and Effects of Lipids

   Författare :David Lindberg; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; atomic force microscopy; fluorescence; Alzheimer’s disease; YOYO-1; amyloid fibrils; lipids; linear dichroism; FRET; circular dichroism; kinetics; Protein aggregation; insulin; thioflavin-T;

   Sammanfattning : Amyloid fibrils are long fibrillar homopolymers of self-assembled proteins. They can be formed by essentially any polypeptide, but are of particular interest because of their occurrence in several incurable and debilitating human diseases, e.g. Alzheimer’s disease (AD). LÄS MER

5. 4. Development of the Amyloid Fibril Characterisation Toolbox - New use for old dyes

   Författare :David Lindberg; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Amyloid-β; Thioflavin-T; Fluorescence spectroscopy; YOYO-1; Amyloid fibrils; Linear dichroism; Alzheimer’s disease;

   Sammanfattning : Amyloid fibrils are self-assembled protein homopolymers that playcentral roles in the pathology of several human diseases, most notably in progressiveneurodegenerative diseases such as Alzheimer’s disease (AD). In AD, fibrils areformed by the amyloid-β (Aβ) peptide, an enzymatic cleavage product that is naturallyproduced in human brain tissue. LÄS MER

7. 5. Development of methods for optical mapping of nanoconfined DNA

   Författare :Lena Nyberg; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; competitive binding; optical mapping; YOYO-1; nanofluidics; antibiotic resistance; fluorescence microscopy; single-molecule; DNA; plasmids; netropsin;

   Sammanfattning : .... LÄS MER