Sökning: "optical mapping"

Visar resultat 1 - 5 av 71 avhandlingar innehållade orden optical mapping.

1. 1. Optical Mapping of Bacterial Plasmids

   Författare :Vilhelm Müller; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; competitive binding; optical mapping; fluorescence microscopy; DNA; plasmids; CRISPR Cas9; antibiotic resistance; nanofluidics; single-molecule;

   Sammanfattning : Bacteria that have acquired resistance to antibiotics represent one of the largest threats to human health and modern health care. The genes encoding resistance are frequently spread via the transfer of plasmids, which are circular double stranded DNA molecules separate from the chromosomal DNA of the bacteria. LÄS MER

3. 2. Methods for barcode analysis in optical DNA mapping

   Författare :Albertas Dvirnas; Beräkningsbiologi och biologisk fysik - Genomgår omorganisation; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA Barcoding; Optical Mapping; Computational Biology;

   Sammanfattning : This thesis is composed of six papers, which all concern different methods and tools used for the analysis of barcodes in nanochannel-based Optical DNA Mapping (ODM). The first four papers consider densely-labeled barcodes while the last two consider sparsely-labeled barcodes. LÄS MER

4. 3. Development of methods for optical mapping of nanoconfined DNA

   Författare :Lena Nyberg; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; competitive binding; optical mapping; YOYO-1; nanofluidics; antibiotic resistance; fluorescence microscopy; single-molecule; DNA; plasmids; netropsin;

   Sammanfattning : .... LÄS MER

5. 4. Competitive-Binding Based Optical DNA Mapping - From Bacterial Plasmids to the Human Genome

   Författare :Vilhelm Müller; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; genetics; single-molecule; nanofluidics; DNA; antibiotic resistance; optical DNA mapping; plasmids; competitive binding; fluorescence microscopy; bacteria;

   Sammanfattning : Significant advances within the field of DNA sequencing have allowed us to study DNA at a level of detail that was previously impossible. However, dynamic genomic regions with a high degree of structural variations, while being linked to disease in humans and increased resistance to antibiotics, are still challenging to characterize. LÄS MER

7. 5. Hydrological Modelling, Topographical Influence and Yield Mapping in Precision Agriculture

   Författare :Andreas Persson; Institutionen för naturgeografi och ekosystemvetenskap; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Spatially distributed hydrological modelling; Precision Agriculture; Yield mapping; Topographical wetness index; Drainage area; Lantbrukshydrologi; marklära; agricultural hydrology; Soil Science;

   Sammanfattning : The use of topographical data in hydrological and agricultural applications has increased with GPS data availability and the concept of precision agriculture. This thesis investigates the relationships between topography and in one case simulated soil moisture and in the other case yield. LÄS MER