Sökning: "Ribonuclease A"

Visar resultat 1 - 5 av 20 avhandlingar innehållade orden Ribonuclease A.

1. 1. Poly(A)-specific Ribonuclease (PARN) : Structural and Functional Studies of Poly(A) Recognition and Degradation

   Författare :Niklas Henriksson; Anders Virtanen; Marvin Wickens; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; Cell- och molekylärbiologi; Molecular Biotechnology; Molekylär bioteknik;

   Sammanfattning : Regulation of mRNA degradation is a powerful way for the cell to regulate gene expression. A critical step in eukaryotic mRNA degradation is the removal of the poly(A) tail at the 3'-end of the mRNA. LÄS MER

3. 2. Poly(A)-Specific Ribonuclease (PARN)

   Författare :Yan-Guo Ren; Eric Westhof; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; Poly A -specific ribonuclease; PARN; deadenylation; processive; oligomeric; cap interacting; aminoglycoside; active site; FE2 -mediated cleavage; metal ion switch; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi; Molekylär cellbiologi; Molecular Cellbiology;

   Sammanfattning : Degradation of the mRNA 3'-end located poly(A) tail is an important step for mRNA decay in mammalian cells. Thus, to understand mRNA decay in detail, it is important to identify the catalytic activities involved in degrading poly(A). LÄS MER

4. 3. Distal to Proximal—Functional Coupling in RNase P RNA-mediated Catalysis

   Författare :Shiying Wu; Leif A. Kirsebom; Britt-Marie Sjöber; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Ribozyme; RNase P; Induced fit model; tRNA progressing; Substrate interaction;

   Sammanfattning : RNase P is a ubiquitous ribonuclease responsible for removing the 5’ leader of tRNA precursor. Bacterial RNase P contains one RNA (RPR) and one protein (RPP) subunit. However, the number of protein variants depends on the origin. The RNA subunit is the catalytic subunit that in vitro cleaves its substrate with and without the protein subunit. LÄS MER

5. 4. PARN - A Tale of A de-Tailor : Functional importance of poly(A) degradation in developmental and telomere biology disorders

   Författare :Sethu Madhava Rao Gunja; Anders Virtanen; Jonas von Hofsten; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; PARN; Telomere biology disorders; snoRNAs; scaRNAs; TR TERC; Zebrafish; Biology with specialization in Molecular Cell Biology; Biologi med inriktning mot molekylär cellbiologi; Molekylär genetik; Molecular Genetics; Molekylär cellbiologi; Molecular Cellbiology;

   Sammanfattning : Poly(A)-specific ribonuclease (PARN) is a eukaryotic 3’-5’exoribonuclease that removes poly(A) tails of many coding and non-coding RNAs. In this thesis, we have studied the physiological role of PARN. LÄS MER

7. 5. Methods for the Synthesis of PET Tracers and NMR Studies of Ribonuclease A

   Författare :Linda Samuelsson; Bengt Långström; Sharon Stone-Elander; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Organic chemistry; [11C]methyl iodide; Stille reaction; palladium; Ribonuclease A; osmolytes; PFG-NMR; Organisk kemi; Organic chemistry; Organisk kemi;

   Sammanfattning : This thesis contains two parts.In the first part, general and versatile palladium-mediated 11C-C bond forming reactions for use in the production of radiotracers for Positron Emission Tomography (PET) were explored. LÄS MER