Sökning: "osmolytes"

Visar resultat 1 - 5 av 13 avhandlingar innehållade ordet osmolytes.

  1. 1. Rate Limiting Factors For Protein Folding

    Författare :Maria Berggård Silow; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; kinetic competition; transient aggregation; diffusion control; reconfiguration time; collapse; osmolytes; broad barriers; Hammond behaviour; two-state; U1A; CI2; Proteins; enzymology; Proteiner; enzymologi;

    Sammanfattning : Abstract. This thesis describes factors that are rate limiting for the folding of two small proteins, U1A and CI2 which fold without accumulating intermediates. The [GdnHCl] dependencies of the unfolding- and refolding kinetics of U1A display downward curvatures. LÄS MER

  3. 2. Methods for the Synthesis of PET Tracers and NMR Studies of Ribonuclease A

    Författare :Linda Samuelsson; Bengt Långström; Sharon Stone-Elander; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Organic chemistry; [11C]methyl iodide; Stille reaction; palladium; Ribonuclease A; osmolytes; PFG-NMR; Organisk kemi; Organic chemistry; Organisk kemi;

    Sammanfattning : This thesis contains two parts.In the first part, general and versatile palladium-mediated 11C-C bond forming reactions for use in the production of radiotracers for Positron Emission Tomography (PET) were explored. LÄS MER

  4. 3. Advancements in Firefly Luciferase-Based Assays and Pyrosequencing Technology

    Författare :Jonas Eriksson; KTH; []
    Nyckelord :bioluminescence; osmolytes; glycine betaine; thermostability; firefly luciferase; inorganic pyrophosphatase; inorganic pyrophosphate; Pyrosequencing technology; secondary DNA-structures; Sequenase; Klenow-polymerase; reaction rates; temperature;

    Sammanfattning : Pyrosequencing is a new DNA sequencing method relying on thesequencing-by-synthesis principle and bioluminometric detectionof nucleotide incorporation events. The objective of thisthesis was improvement of the Pyrosequencing method byincreasing the thermal stability of firefly luciferase, and byintroducing an alternative DNA polymerase and a new nucleotideanalog. LÄS MER

  5. 4. Self-Assembly in Lipid-Protein Systems : Lung Surfactant, Stratum Corneum and Model Membranes

    Författare :Jenny Andersson; Fysikalisk kemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Lipid self-assembly; lung surfactant; stratum corneum; dehydrin; cholesterol; osmolytes; urea; TMAO; ssNMR; cSAXS;

    Sammanfattning : This thesis explores lipid self-assembly and aims to give a broad picture of self-assembly structures in simple and complex lipid-protein systems. The systems studied are lung surfactant, stratum corneum and simple model membranes. The lung surfactant mixture lines the alveolus in our lungs and stabilises the air- tissue interface. LÄS MER

  7. 5. Roles of intermolecular interactions in amyloid fibril formation mechanisms

    Författare :Tony Werner; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; disulfide-bonds; Atox1; α-synuclein; Aggregation; protein interactions; Parkinson’s disease; β-parvalbumin; macromolecular crowding; osmolytes; Amyloid fibrils;

    Sammanfattning : Amyloid fibrils, a major pathological feature of several neurodegenerative disorders, are highly stable, insoluble aggregates of misfolded proteins. The formation of such aggregates involves a complex equilibrium between protein monomers, different on- and off-pathway transient oligomeric species, and amyloid fibrils. LÄS MER