Sökning: "Recombinant protein production"

Visar resultat 21 - 25 av 188 avhandlingar innehållade orden Recombinant protein production.

1. 21. Systems Biology of Recombinant Protein Production by Fungi

   Författare :Lifang Liu; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

   Sammanfattning : Systems biology has emerged as a highly potent tool for studying biological processes over the last decades. However, its application to complex metabolic processes such as protein secretion is still at the infant stage. LÄS MER

3. 22. Production and folding of proteins in the periplasm of Escherichia coli

   Författare :Rageia Elfageih; Jan-Willem de Gier; Hans-Georg Koch; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; periplasm; recombinant protein production; disulfide bond containing proteins; translation initiation region; protein translocation machinery; co-translational folding; neuraminidases; biokemi; Biochemistry;

   Sammanfattning : The Gram-negative bacterium E. coli is the most widely used host for the production of recombinant proteins. Disulfide bond containing recombinant proteins are usually produced in the periplasm of E. coli since in this compartment of the cell - in contrast to the cytoplasm - disulfide bond formation is promoted. LÄS MER

4. 23. Methodology for high-throughput production of soluble recombinant proteins in Escherichia coli

   Författare :Katrin Markland; Gen Larsson; Pär Nordlund; Jenny Ottosson; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; high-throughput methodology; recombinant protein production; solubility; productivity; quality; cultivation technology; parallel reactors; Biochemistry; Biokemi;

   Sammanfattning : The aim of this work was to investigate and determine central parameters that can be used to control and increase the solubility, quality and productivity of recombinant proteins. These central parameters should be applicable under the constraints of high-throughput protein production in Escherichia coli. LÄS MER

5. 24. Vitamin K-dependent protein S - studies of synthesis, structure and function

   Författare :Sofia Somajo; Malmö Klinisk kemi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; C4b-binding protein; coagulation; microparticles; protein C; protein S; structure; tissue factor pathway inhibitor;

   Sammanfattning : This thesis is based on four projects with the overall aim to investigate vitamin K-dependent protein S functions in relation to its structure. The anticoagulant functions of protein S can be divided in three: 1) cofactor for activated protein C (APC), 2) cofactor for tissue factor pathway inhibitor (TFPI) and 3) APC and TFPI-independent anticoagulant functions. LÄS MER

7. 25. Impact of glucose uptake rate on recombinant protein production in Escherichia coli

   Författare :Emma Bäcklund; Gen Larsson; Olle Holst; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; AIDA-autotransporter; Escherichia coli; fed-batch; glucose uptake rate; integral membrane proteins; outer membrane proteins; periplasmic retention; phosphotransferase system; recombinant proteins; specific growth rate; surface expression; Biology; Biologi;

   Sammanfattning : Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. LÄS MER