Sökning: "Recombinant Proteins chemistry"

Visar resultat 6 - 10 av 18 avhandlingar innehållade orden Recombinant Proteins chemistry.

1. 6. Analysis of noncovalent and covalent protein-ligand complexes by electrospray ionisation mass spectrometry

   Författare :Gustav Sundqvist; Harry Brumer; Per Andrén; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; noncovalent complex; droplet fission; nano-electrospray ionisation; mass spectrometry; Analytical chemistry; Analytisk kemi;

   Sammanfattning : In this thesis, the application of electrospray ionisation mass spectrometry (ESI-MS) to the analysis of intact proteins is demonstrated. In papers I and II, the use of ESI-MS for the analysis of noncovalent protein-ligand complexes were discussed. LÄS MER

3. 7. Ribosomes and subunits from Escherichia coli studied by asymmetrical flow field-flow fractionation

   Författare :Mikael Nilsson; Centrum för analys och syntes; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; metabolic engineering; VHb; Vitreoscilla hemoglobin; E. coli; Escherichia coli; separation; subunits; ribosomes; Asymmetrical flow field-flow fractionation; flow FFF; optimization; biotechnological production; Analytical chemistry; Analytisk kemi;

   Sammanfattning : In this thesis asymmetrical flow field-flow fractionation (FFF) separations of ribosomes and subunits have been studied. Ribosomal material was prepared from Escherichia coli (E. coli) cells and analyzed by the size separation technique. LÄS MER

4. 8. Studies on a serine peptidase inhibitor locus on human chromosome 20 Characterization of novel genes encoding proteins with WFDC and kunitz domains

   Författare :Adam Clauss; Malmö Klinisk kemi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Medicin människa och djur ; Medicine human and vertebrates ; kunitz; Protase inhibitor; WFDC;

   Sammanfattning : An unlimited number of proteolytic reactions are carried out by enzymes in biological systems. These enzymes are a necessity of life, but they can also be dangerous if they are not controlled. LÄS MER

5. 9. Capturing transient peptide assemblies associated with Alzheimer's disease : Native mass spectrometry studies of amyloid-β oligomerization

   Författare :Nicklas Österlund; Astrid Gräslund; Leopold L. Ilag; Mikael Oliveberg; Sheena E. Radford; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Protein aggregation; amyloid-β; neurodegeneration; Alzheimer’s disease; chaperones; DnaJ; biomembranes; native mass spectrometry; Biophysics; biofysik;

   Sammanfattning : Correct folding of proteins is essential for maintaining a functional living cell. Misfolding and aggregation of proteins, where non-native intermolecular interactions form large and highly ordered amyloid aggregates with low free energy, is hence associated with multiple diseases. LÄS MER

7. 10. Apolipoprotein M – Studies of Structure and Function

   Författare :Josefin Ahnström; Malmö Klinisk kemi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; signal peptidase; signal peptide; crystallization; Apolipoprotein; lipocalin; HDL; coronary heart disease;

   Sammanfattning : Apolipoprotein M (apoM), a 25 kDa plasma protein, is found in all major lipoprotein classes, although the majority is found in high density lipoproteins (HDL). ApoM has been suggested to be involved in the formation of and adding to the antiatherogenic functions of HDL, but its function is still not completely known. LÄS MER