Sökning: "Pyrosequencing®"

Visar resultat 21 - 25 av 106 avhandlingar innehållade ordet Pyrosequencing®.

  1. 21. Lactobacillus iners and the normal vaginal flora

    Författare :Tell Jakobsson; Urban Forsum; Lars Fåhraeus; Per-Göran Larsson; Ingegerd Adlerberth; Linköpings universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Vagina; microbes; lactobacilli; phenotypic methods; molecular biology; 16S rRNA; pyrosequencing; Clinical bacteriology; Klinisk bakteriologi;

    Sammanfattning : The ecological niche of the vagina contains a large number of different microbes that are constantly interacting with each other and the host. Culture methods have not been sufficient in order to resolve the complexity of the normal vaginal flora. LÄS MER

  3. 22. Molecular Signatures of Cancer

    Författare :Esther Edlundh-Rose; Joakim Lundeberg; Peter Södervist; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; : BRAF; NRAS; MGMT; methylation; pyrosequencing; microarray technology; gene expression analysis; Molecular biology; Molekylärbiologi;

    Sammanfattning : Cancer is an important public health concern in the western world, responsible for around 25% of all deaths. Although improvements have been made in the diagnosis of cancer, treatment of disseminated disease is inefficient, highlighting the need for new and improved methods of diagnosis and therapy. LÄS MER

  4. 23. Interrogation of Nucleic Acids by Parallel Threading

    Författare :Erik Pettersson; Joakim Lundeberg; Afshin Ahmadian; Ann-Christine Syvänen; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; genotyping; multiplex amplification; trinucleotide threading; single nucleotide polymorphism; genotype calling; Expectation-Maximization; protein-epitope signature tag; expression profiling; Human Protein Atlas; pooled genomic DNA; Pyrosequencing; 454; allelotyping; association studies; bioinformatics; Bioengineering; Bioteknik;

    Sammanfattning : Advancements in the field of biotechnology are expanding the scientific horizon and a promising era is envisioned with personalized medicine for improved health. The amount of genetic data is growing at an ever-escalating pace due to the availability of novel technologies that allow massively parallel sequencing and whole-genome genotyping, that are supported by the advancements in computer science and information technologies. LÄS MER

  5. 24. Molecular Tools for Nucleic Acid Analysis

    Författare :Deirdre O'Meara; KTH; []
    Nyckelord :nucleic acid capture; modular probes; biosensor; bio-magnetic separation; hepatitis C; sequencing; pyrosequencing; mutation detection; HIV-1; drug resistance; SNP; allele-specific extension; apyrase; genotyping.;

    Sammanfattning : Nucleic acid technology has assumed an essential role invarious areas ofin vitrodiagnostics ranging from infectious diseasediagnosis to human genetics. An important requirement of suchmolecular methods is that they achieve high sensitivity andspecificity with a fast turnaround time in a cost-effectivemanner. LÄS MER

  7. 25. Genetic variation in the folate receptor-alpha and methylenetetrahydrofolate reductase genes as determinants of plasma homocysteine concentrations

    Författare :Anna Böttiger; Torbjörn Nilsson; Henrik Zetterberg; Örebro universitet; []
    Nyckelord :LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; Folate receptor-alpha; Folate; FOLR1; Haplotypes; Homocysteine; Methylenetetrahydrofolate reductase; MTHFR; Polymorphisms; Pyrosequencing®; SSCP; Medicine; Medicin; Biomedicin; Biomedicine;

    Sammanfattning : Elevated total plasma homocysteine (tHcy) is a risk factor for cardiovascular disease and neurocognitive disease such as dementia. The B vitamins folate and B12 are the main de terminants of tHcy. tHcy concentration can also be affected by mutations in genes coding for receptors, enzymes and transporters important in the metabolism of Hcy. LÄS MER