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Visar resultat 1 - 5 av 57 avhandlingar som matchar ovanstående sökkriterier.

1. 1. Tools for the development of intensified perfusion processes for mammalian cell culture

   Författare :Hubert Schwarz; Véronique Chotteau; Mark Smales; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Recombinant protein biomanufacturing; Perfusion process; Integrated continuous bioprocessing; Monoclonal antibody; Erythropoietin; Chinese Hamster Ovary cells; Human Embryonic Kidney cells; Perfusion medium optimization; Cell specific perfusion rate; Raman spectroscopy; N-glycosylation; Microbioreactors; Design of Experiments; Biotechnology; Bioteknologi;

   Sammanfattning : Recombinant protein therapeutics have become an indispensable part of modern medicine to treat a wide variety of diseases. Their manufacture is mostly based on mammalian cells with fed-batch production bioreactors and downstream batch purification unit operations. LÄS MER

3. 2. Hydrodynamics considerations in cells systems from ocean flow to perfusion cultivation process

   Författare :Caijuan Zhan; Véronique Chotteau; Jeffrey Chalmers; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Microorganism; cells; hydrodynamics; turbulence; numerical simulation; CFD; Resonance; Wave bioreactor; ATF; TFF; perfusion; design feeding strategy; Bioteknologi; Biotechnology;

   Sammanfattning : Microorganisms and animal cells are grown surrounded by fluid, which is providing them with nutrients and removing their waste products. In nature and industry processes, cells/microbes can be subject to aggressive environments, such as turbulent flow or shear flow. LÄS MER

4. 3. High cell density perfusion process development for antibody producing Chinese Hamster Ovary cells

   Författare :Ye Zhang; Vincent Bulone; Veronique Chotteau; Carl-Fredrik Mandenius; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; WAVE Bioreactor; Cellbag; ATF; TFF; hollow fiber; CSPR; CellTank; SUB; single-use-bioreactor; disposable; perfusion rate; Biotechnology; Bioteknologi;

   Sammanfattning : Perfusion operation mode is currently under fast expansion in mammalian cell based manufacturing of biopharmaceuticals, not only for labile drug protein but also for stable proteins such as monoclonal antibodies (mAbs). Perfusion mode can advantageously offer a stable cell environment, long-term production with high productivity and consistent product quality. LÄS MER

5. 4. Ventilation/Perfusion Matching and its Effect on Volatile Pharmacokinetics

   Författare :Moritz Andreas Kretzschmar; Anders Larsson; Jan Jakobsson; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Anesthesia; Animal models in research; Mass spectrometry; Gas chromatography; MIGET; Ventilation-perfusion; Desflurane; Isoflurane; Bronchoconstriction; Anestesiologi och intensivvård; Anaesthesiology and Intensive Care;

   Sammanfattning : The mismatching of alveolar ventilation and perfusion (VA/Q) is the major determinant of impaired gas exchange. The gold standard for analyzing VA/Q distribution is the multiple inert gas elimination technique (MIGET), conventionally based on gas chromatography (GC), and, although simple in principle, a technically demanding procedure limiting its use. LÄS MER

7. 5. Quantification of pulmonary scintigraphy

   Författare :Jiahua Xu; Göteborgs universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Emphysema; Inhomogeneity; Perfusion; scintigraphy; SPECT; 99mTc-Technegas; Ventilation; V Q;

   Sammanfattning : Modern nuclear medicine techniques, such as SPECT (single photon emission computed tomography), enable quantification and localisation of lung function in small lung elements, but appropriate methods are not yet available. The aim of the present project was therefore to develop and evaluate methods for mapping lung function in planar scintigrams and in SPECT. LÄS MER