Sökning: "Mikael Widersten"

Visar resultat 6 - 10 av 21 avhandlingar innehållade orden Mikael Widersten.

1. 6. Role of Multiple Glutathione Transferases in Bioactivation of Thiopurine Prodrugs : Studies of Human Soluble Glutathione Transferases from Alpha, Kappa, Mu, Omega, Pi, Theta, and Zeta Classes

   Författare :Birgitta I. Eklund; Bengt Mannervik; Mikael Widersten; Ralf Morgenstern; Uppsala universitet; []
   Nyckelord :Biochemistry; Glutathione Transferases; Thiopurines; Prodrug; Bioactivation; Screening Method; Chemotherapy; Functional plasticity; Modulated activity; Biokemi;

   Sammanfattning : A screening method was developed for identification of catalytically active enzymes in combinatorial cDNA libraries of mutated glutathione transferase (GST) derivatives expressed in E. coli. The method is based on spraying monochlorobimane (MCB) directly over bacterial colonies growing on agar. LÄS MER

3. 7. Engineered Alcohol Dehydrogenases for Stereoselective Chemical Transformations

   Författare :Thilak Reddy Enugala; Mikael Widersten; Manfred T. Reetz; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; alcohol dehydrogenase-A; biocatalysts; protein engineering; enzyme kinetics; sec-alcohols; ketones; stereoselectivity; regioselectivity; substrate selectivity and promiscuity.; Biokemi; Biochemistry;

   Sammanfattning : Enzymes are biomolecules built from amino acids and catalyze the chemical transformations in a cell. Enzymes are by nature stereoselective, biodegradable, environmentally friendly, and can perform catalysis in aqueous solutions and at ambient temperatures. LÄS MER

4. 8. Protease Activity, Inhibition and Ligand Interaction Analysis : Developments and Applications for Drug Discovery

   Författare :Thomas Gossas; Helena Danielson; Mikael Widersten; Alexander Wlodawer; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; proteases; kinetics; biosensors; inhibition; calcium; drug discovery; Biokemi;

   Sammanfattning : The present study has focused on characterising protease-ligand interactions in the context of drug discovery. The proteases that have been studied are human matrix metallopeptidase 12 (MMP-12), HIV-protease and Hepatitis C virus (HCV) NS3/NS4A protease. LÄS MER

5. 9. Biochemical Studies on a Plant Epoxide Hydrolase : Discovery of a Proton Entry and Exit Pathway and the Use of In vitro Evolution to Shift Enantioselectivity

   Författare :Ann Gurell; Mikael Widersten; Bengt Mannervik; Dick Janssen; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; epoxide hydrolase; enantioselectivity; in vitro evolution; proton wire; epoxides; selectivity; CASTing; structure-function; Biochemistry; Biokemi; Biokemi; Biochemistry;

   Sammanfattning : The work leading to this thesis has provided additional information and novel knowledge concerning structure-function relationship in the potato epoxide hydrolase. Epoxide hydrolases are enzymes catalyzing the hydrolysis of epoxides to yield the corresponding vicinal diols. LÄS MER

7. 10. Characterization and Directed Evolution of an Alcohol Dehydrogenase : A Study Towards Understanding of Three Central Aspects of Substrate Selectivity

   Författare :Emil Hamnevik; Mikael Widersten; Gerrit Poelarends; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Alcohol dehydrogenase; ADH-A; Biocatalysis; Directed evolution; Enantioselectivity; Enzyme kinetics; Enzyme mechanisms; Protein Engineering; Regioselectivity; Substrate selectivity; Biokemi; Biochemistry;

   Sammanfattning : Many different chemicals are used in the everyday life, like detergents and pharmaceuticals. However, their production has a big impact on health and environment as much of the raw materials are not renewable and the standard ways of production in many cases includes toxic and environmentally hazardous components. LÄS MER