Sökning: "Birgitta I. Eklund"

Hittade 1 avhandling innehållade orden Birgitta I. Eklund.

1. 1. Role of Multiple Glutathione Transferases in Bioactivation of Thiopurine Prodrugs : Studies of Human Soluble Glutathione Transferases from Alpha, Kappa, Mu, Omega, Pi, Theta, and Zeta Classes

   Författare :Birgitta I. Eklund; Bengt Mannervik; Mikael Widersten; Ralf Morgenstern; Uppsala universitet; []
   Nyckelord :Biochemistry; Glutathione Transferases; Thiopurines; Prodrug; Bioactivation; Screening Method; Chemotherapy; Functional plasticity; Modulated activity; Biokemi;

   Sammanfattning : A screening method was developed for identification of catalytically active enzymes in combinatorial cDNA libraries of mutated glutathione transferase (GST) derivatives expressed in E. coli. The method is based on spraying monochlorobimane (MCB) directly over bacterial colonies growing on agar. LÄS MER