Sökning: "Lignocellulosic hydrolysates"

Visar resultat 1 - 5 av 22 avhandlingar innehållade orden Lignocellulosic hydrolysates.

  1. 1. Fermentation of Lignocellulosic Hydrolysates: Inhibition and Detoxification

    Författare :Eva Palmqvist; Biologiska institutionen; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; fermentation strategies; mechanisms; identification; inhibition; lignocellulosic hydrolysates; Ethanol; Saccharomyces cerevisiae; virology; virologi; mykologi; mycology; Mikrobiologi; bakteriologi; bacteriology; Microbiology;

    Sammanfattning : The ethanol yield and productivity obtained during fermentation of lignocellulosic hydrolysates is decreased due to the presence of inhibiting compounds, such as weak acids, furans and phenolic compounds produced during hydrolysis. Evaluation of the effect of various biological, physical and chemical detoxification treatments by fermentation assays using Saccharomyces cerevisiae was used to characterise inhibitors. LÄS MER

  3. 2. CRISPRi/a for investigating yeast tolerance to inhibitors in lignocellulosic hydrolysates

    Författare :Ibai Lenitz Etxaburu; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Lignocellulosic hydrolysates; ChIP-exo; Screening; Biosensor; Formic acid; Competitive growth assay; Yeast; Tolerance; CRISPRi a; Acetic acid;

    Sammanfattning : Saccharomyces cerevisiae has immense potential as a cell factory in various biotechnological processes where biomass from agricultural industry residues is used as feedstock. Nonetheless, the inhibitors released during the pretreatment of the biomass makes lignocellulosic hydrolysates a challenging substrate for microorganisms. LÄS MER

  4. 3. Exploring Saccharomyces cerevisiae’s responses to acetic acid and other inhibitors found in lignocellulosic hydrolysates

    Författare :Maurizio Mormino; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; acetic acid biosensor; formic acid; CRISPRi screening; acetic acid; Saccharomyces cerevisiae; lignocellulosic inhibitor tolerance; differential gene expression;

    Sammanfattning : The limited tolerance of the budding yeast Saccharomyces cerevisiae to lignocellulosic hydrolysate inhibitors is a key challenge to its use in biorefinery cell factories. Considerable resources have been invested in the isolation of yeast strains with better tolerance towards the inhibitors released during lignocellulose hydrolysis, such as acetic acid. LÄS MER

  5. 4. Improving the Response of Saccharomyces cerevisiae to Lignocellulosic Hydrolysate Inhibitors in Ethanolic Fermentation

    Författare :Joao Almeida; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; furfural; ethanol; fermentation; phenolics; furaldehyde; HMF; ADH1; xylose reductase; tolerance; Saccharomyces cerevisiae; Lignocellulosic hydrolysate inhibitors; ADH6;

    Sammanfattning : The production of ethanol based on lignocellulosic biomass requires the fermentation of a hydrolysate containing hexose and pentose sugars in an inhibitory environment. In fact, the lignocellulosic hydrolysate obtained from pretreatment and hydrolysis of the raw material contains a variety of inhibitory compounds, including (i) the furaldehydes 5-hydroxymethyl-2-furaldehyde (HMF) and 2-furaldehyde (furfural), (ii) weak acids (e. LÄS MER

  7. 5. Short-term adaptation of S. cerevisiae to lignocellulosic inhibitors: Underlying metabolic and physiological changes

    Författare :Marlous van Dijk; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; lignocellulosic inhibitor tolerance; second generation bioethanol; short-term adaptation; differential gene expression; industrial yeast strains;

    Sammanfattning : The limited tolerance of Saccharomyces cerevisiae (budding yeast) to inhibitors present in lignocellulosic hydrolysates is a major challenge in second-generation bioethanol production. Short-term adaptation of the yeast to lignocellulosic hydrolysates during cell propagation has been shown to improve its tolerance, and thus its performance in lignocellulose fermentation. LÄS MER