Sökning: "Heterologous expression"

Visar resultat 16 - 20 av 99 avhandlingar innehållade orden Heterologous expression.

  1. 16. Redesign of Alpha Class Glutathione Transferases to Study Their Catalytic Properties

    Författare :Lisa O Nilsson; Ralf Morgenstern; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; glutathione transferase; heterologous expression; heterodimer; hydroxyalkenal; protein redesign; sequential design; dimer-interface interactions; enzyme kinetics; glutathione; dynamic C-terminus; viscosity dependence; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : A number of active site mutants of human Alpha class glutathione transferase A1-1 (hGST A1-1) were made and characterized to determine the structural determinants for alkenal activity. The choice of mutations was based on primary structure alignments of hGST A1-1 and the Alpha class enzyme with the highest alkenal activity, hGST A4-4, from three different species and crystal structure comparisons between the human enzymes. LÄS MER

  3. 17. Cloning and characterization of three Family 9 cellulases from plants and fungi

    Författare :Ulla Rudsander; KTH; []
    Nyckelord :endglucanase; hybrid aspen; cellulose biosynthesis; white rot fungus; homology model; heterologous expression; transcript analysis;

    Sammanfattning : .... LÄS MER

  4. 18. Heterologous expression, characterization and applications of carbohydrate active enzymes and binding modules

    Författare :Åsa Kallas; Tuula T. Teeri; Edward A. Bayer; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; Populus; xyloglucan endotransglycosylase; carbohydrate binding modules; endo- 1; 4 -b-xylanase; Escherichia coli; Pichia pastoris; N-glycosylation; fiber analysis; Bioengineering; Bioteknik; Wood fibre and forest products; Träfiber- och virkeslära;

    Sammanfattning : Wood and wood products are of great economical and environmental importance, both in Sweden and globally. Biotechnology can be used both for achieving raw material of improved quality and for industrial processes such as biobleaching. LÄS MER

  5. 19. In vitro and in vivo approaches in the characterization of XTH gene products

    Författare :Nomchit Kaewthai; Harry Brumer; Jocelyn Rose; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Arabiodopsis thaliana; Hordeum vulgare; plant cell wall; xyloglucan; glycoside hydrolase family 16; xyloglucan endo-transglycosylase hydrolase gene family; xyloglucan endo-hydrolase; heterologous protein expression; Pichia pastoris; T-DNA insertion; in situ XET XEH assay; high-througput screening; Plant physiology; Växtfysiologi; Biochemistry; Biokemi; Functional genomics; Funktionsgenomik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : ABSTRACT The xyloglucan endo-transglycosylase/hydrolase (XTH) genes are found in all vascular and some nonvascular plants. The XTH genes encode proteins which comprise a subfamily of glycoside hydrolase (GH) family 16 in the Carbohydrate-Active enZYmes (CAZY) classification. LÄS MER

  7. 20. Expression and synthetic activation of [FeFe]-hydrogenases in cyanobacteria

    Författare :Adam Wegelius; Peter Lindblad; Karin Stensjö; Namita Khanna; Matthew Posewitz; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Chemistry with specialization in Microbial Chemistry; Kemi med inriktning mot mikrobiell kemi;

    Sammanfattning : Photosynthetic microbes can be utilized for hydrogen production, generating a clean, carbon neutral energy carrier from abundant substrates. Cyanobacteria are photosynthetic prokaryotes with large potential for biotechnological energy applications and several strains are capable of hydrogen production. LÄS MER