Sökning: "Heterologous expression"

Visar resultat 21 - 25 av 98 avhandlingar innehållade orden Heterologous expression.

  1. 21. Plant Aquaporins: A study of expression, localization, specificity, and regulation

    Författare :Sara Sjövall Larsen; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Växtbiokemi; Plant biochemistry; phosphorylation; protein kinase; overexpression; NIP; MIP; plant; aquaporin;

    Sammanfattning : Aquaporins, or Major Intrinsic Proteins (MIPs), are integral proteins that facilitate transport of water and other small neutral solutes across biological membranes. They belong to a well conserved and ancient family of proteins, present in all organisms ranging from bacteria to plants and humans. LÄS MER

  3. 22. Rational and combinatorial genetic engineering approaches for improved recombinant protein production and purification

    Författare :Nina Bandmann; Per-Åke Nygren; Helena Berglund; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Aqueous two-phase system; combinatorial library; expression vector; flow cytometry; fusion tag; partitioning; peptide library; phage display; recombinant proteins; ribosomal binding site; translation initiation; Bioengineering; Bioteknik;

    Sammanfattning : The bacterium Escherichia coli (E. coli) is in many situations an ideal host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve sufficiently high product quantities. LÄS MER

  4. 23. Functional genomics at the interface of protein expression and biophysical analysis

    Författare :Xiaoqiu Wu; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Protein characterization is central to any functional genomics effort, hence protein expression and purification play fundamental roles in these initiatives. In this study strategies for protein expression in E. coli were analysed, and several parameters were investigated respect to high-level expression of functional proteins. LÄS MER

  5. 24. In vitro and in vivo oxidation of diastereomers of lignin models of arylgylcerol b-aryl ether type and heterologous expression of laccase

    Författare :Christina Bohlin; Leif Jönsson; Ming Tien; Karlstads universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; Kemi; Chemistry;

    Sammanfattning : Enzymic and non-enzymic oxidation of lignin models of the arylglycerol β-aryl ether type was investigated in experiments aimed at providing a better understanding of the initial phase of biological lignin degradation. Product profiles and diasteromer selectivity were determined to gain knowledge of the properties of the oxidants and pave the way for evaluation of the presence of the oxidants in ligninolytic fungal cultures. LÄS MER

  7. 25. Thermostable Phytase from a Bacillus sp.: Heterologous Production, Mutation, Characterization and Assay Development

    Författare :Tran Thi Thuy; Bioteknik; []
    Nyckelord :ß-propeller phytase; recombinant protein; site-directed mutagenesis; phytate;

    Sammanfattning : Phytase is an important enzyme in the food/feed industry. It catalyzes the hydrolysis of phytate, an anti-nutrient compound present in cereals and grains, to release orthophosphate and myo-inositol-6-phosphate with lower degrees of phosphorylation. LÄS MER