Sökning: "Glutathione transferase"

Visar resultat 6 - 10 av 85 avhandlingar innehållade orden Glutathione transferase.

  1. 6. The Quest for Functional Quasi-Species in Glutathione Transferase Libraries

    Författare :Arna Rúnarsdóttir; Bengt Mannervik; Ralf Morgenstern; Uppsala universitet; []
    Nyckelord :glutathione transferase; directed evolution; multivariate analysis; quasi-species; isothiocyanates; Biokemi; Biochemistry;

    Sammanfattning : Glutathione transferases (GSTs) are good candidates for investigations of enzyme evolution, due to their broad substrate specificities and structural homology. The primary role of GSTs is to act as phase II detoxifying enzymes protecting the cell from toxic compounds of both endo- and exogenous origins. LÄS MER

  3. 7. Structure-Function Relationships of Pi Class Glutathione Transferase Studied by Protein Engineering

    Författare :Usama M. Hegazy; Bengt Mannervik; Mikael Widersten; T. J. Mantle; Uppsala universitet; []
    Nyckelord :Biochemistry; Glutathione transferase; targeted chemical modification; lock-and-key motif; cooperativity; stucture-function relationship; protein engineering; unnatural amino acid; site-specific mutation; Biokemi;

    Sammanfattning : The glutathione transferases (GSTs) represent a superfamily of dimeric proteins involved in cellular detoxication by catalyzing the nucleophilic addition of the reduced glutathione (GSH) to the hydrophobic electrophiles. The present work focuses on the functional role of the conserved structures of GSTP1-1. LÄS MER

  4. 8. Studies on hepatic glutathione transferases from rat and man : 1. A kinetic mechanism for glutathione transferase A from rat liver. 2. Transferase µ, a new form of glutathione transferase in human liver

    Författare :Margareta Warholm; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : This study of glutathione transferases was undertaken to achieve a better understanding of the catalytic and molecular properties of various isozymes of this group of drug-metabolizing enzymes.The steady-state kinetics of purified glutathione transferase A from rat liver with glutathione (GSH) and 1,2-dichloro- 4-nitrobenzene as substrates, did not follow a simple Michaelis- Menten equation. LÄS MER

  5. 9. Evolutionary Analysis and Posttranslational Chemical Modifications in Protein Redesign : A Study on Mu Class Glutathione Transferases

    Författare :Ylva Ivarsson; Bengt Mannervik; Gun Stenberg; Richard N. Armstrong; Uppsala universitet; []
    Nyckelord :Biochemistry; glutathione transferase; evolutionary analysis; positive selection; epoxide; stereoselectivity; protein modification; Biokemi;

    Sammanfattning : Glutathione transferases (GSTs) constitute a family of multifarious enzymes that conjugate glutathione (GSH) with a wide range of electrophiles. GSTs are grouped into different classes based on protein sequence similarities. Despite high sequence identities between GSTs of the same class they often display different substrate specificites. LÄS MER

  7. 10. Alpha-class Glutathione Transferases from Pig: a Comparative Study

    Författare :Natalia Fedulova; Bengt Mannervik; Francoise Raffalli-Mathieu; Mario Lo Bello; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; glutathione transferase; substrate selectivity; steroidogenesis; Sus scrofa; functional comparison; irreproducibility; reproducible assays; Biochemistry; Biokemi; Biokemi; Biochemistry;

    Sammanfattning : Glutathione transferases (GSTs, EC 2.5.1.18) possess multiple functions and have potential applications in biotechnology. LÄS MER