Sökning: "DNA ligand"

Visar resultat 6 - 10 av 175 avhandlingar innehållade orden DNA ligand.

  1. 6. Biophysical studies of DNA binding – by the large filament-forming protein Rad51 and the small minor-groove binder Hoechst 33258

    Författare :Louise Fornander; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Rad51; Linear Dichroism; DNA; Isothermal Titration Calorimetry.; Spectroscopy; Nanofluidics; Hoechst 33258; DNA-binding ligands;

    Sammanfattning : Mechanistic insight into the nature of DNA-binding ligands is crucial for both drug development as well as understanding more complex biological reactions that take place in the cell. In this Thesis, two rather different DNA-binding molecules are considered: 1. LÄS MER

  3. 7. DNA Interactions with Chiral Polyaza-aromatic Ruthenium(II) Complexes

    Författare :Per Lincoln; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; phenanthroline; binding geometry; binding isotherm; DNA; dppz; linear dichroism; intercalation; ruthenium; cooperative binding;

    Sammanfattning : The binding to DNA of a series of chiral ruthenium complexes is studied with optical spectroscopy techniques, including isotropic absorption, linear dichroism (LD) and emission anisotropy as well as steady-state and time-resolved luminescence. The thesis focuses on the ìlight-switchî complex [Ru(phen)2dppz]2+ which binds to DNA by intercalation and luminesces when bound to DNA but not in pure buffer solution (phen=1,10-phenanthroline; dppz=dipyrido[3,2-a:2',3'-c]phenazine). LÄS MER

  4. 8. Structure and dynamics of DNA : sequence dependent effects and ligand interactions studied by NMR

    Författare :Mikael Leijon; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : .... LÄS MER

  5. 9. Concatemerization of Synthetic Oligonucleotides

    Författare :Lu Sun; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; gel electrophoresis; DNA-binding; QCM-D; DNA concatemer; hybridization; DNA formation; self-assembly;

    Sammanfattning : DNA nanotechnology has become an important research field because of its advantages in high predictability and accuracy of base-paring recognition. For example, the DNA concatemer, one of the simplest DNA constructs in shape, has been used to enhance signals in biosensing. LÄS MER

  7. 10. Chiral Ruthenium Dipyrodiphenazine Complexes: DNA Binding and Excited State Relaxation

    Författare :Björn Önfelt; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; polarization spectroscopy; DNA-probe; dppz; excited-state relaxation; intercalation; solvent relaxation; DNA-threading;

    Sammanfattning : Ruthenium complexes with aromatic ligands have been extensively studied due to their interesting photophysical properties, including photophysics and electron transfer upon interaction with nucleic acids. For example, the broad absorption spectrum makes them suitable as antenna molecules in photochemical reaction systems and their ability to accept or donate electrons in their excited state could have potential in opto-electronical devices. LÄS MER