Sökning: "DNA cleavage"

Visar resultat 16 - 20 av 108 avhandlingar innehållade orden DNA cleavage.

  1. 16. Notch signaling : from receptor cleavage to chromatin remodeling

    Författare :Kia Strömberg; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : One of the principal signaling mechanisms that control development of multicellular organisms is the Notch signaling pathway. The transmembrane Notch receptor, presented at the cell surface, interacts with ligand on neighbouring cells. LÄS MER

  3. 17. Padlock probes : Circularizing oligonucleotides for localized detection of DNA sequence variants

    Författare :Mats Nilsson; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

    Sammanfattning : Present techniques for localized detection of DNA sequence variants have limitations due to problems with sensitivity, specificity, signal localization or ability to perform many analyses simultaneously.In this study a novel gene analytic reagent is presented - the padlock probe. LÄS MER

  4. 18. Improving integration efficiency and precision of CRISPR/Cas9-mediated genome editing

    Författare :Sandra Wimberger; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Genome editing; CRISPR-Cas9; DNA repair; genomic integration; off-targets;

    Sammanfattning : The clustered regularly interspaced short palindromic repeats (CRISPR) – CRISPR-associated protein 9 (Cas9) system has revolutionized the field of genome engineering, providing a cost-effective and fast tool for targeted gene modifications. Endogenous repair pathways, including error-prone non-homologous end joining and alternative end joining, or precise homology-directed repair (HDR), mend Cas9-induced DNA double-strand breaks. LÄS MER

  5. 19. Homing Endonucleases and Horizontal Gene Transfer in Bacteria and Bacteriophages

    Författare :David Nord; Britt-Marie Sjöberg; Steinar Johansen; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; dsDNA cleavage; ribonucleotide reductase; GIY-YIG motif; Molecular biology; Molekylärbiologi; molekylärbiologi; Molecular Biology;

    Sammanfattning : Homing endonuclease genes (HEGs) are selfish genetic elements that mediate their own super-Mendelian inheritance. This is mediated by the homing endonuclease cleavage of a HEG- allele followed by recombination-repair with a HEG+ allele.The majority of the HEGs are encoded in intervening sequences (IVSs). LÄS MER

  7. 20. Genetic organization of encapsulated and non-encapsulated Haemophilus influenzae strains. Analysis of the glpTQ and 23S rRNA operons

    Författare :Xin-Ming Song; Malmö Klinisk mikrobiologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Microbiology; intervening sequence; 23S rRNA; transcription; DNA sequence; glpT; Haemophilus influenzae; glpQ; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : Encapsulated Haemophilus influenzae type b (Hib) and non-encapsulated H. influenzae (NTHi) are important pathogens causing invasive and mucosal infections in children. A 42 kDa surface-exposed lipoprotein, protein D, has been found to be a potential virulence factor of NTHi. LÄS MER