Sökning: "CRISPR-Cas9"
Visar resultat 1 - 5 av 73 avhandlingar innehållade ordet CRISPR-Cas9.
1. Modeling Human Hematopoiesis Using the CRISPR/Cas9 System
Sammanfattning : Hematopoietic stem cells (HSCs) have the ability to self-renew and to give rise to all blood cells of the different lineages, and are thereby responsible for the replenishment of blood cells throughout life. These cells are tightly regulated by extrinsic and intrinsic regulators, such as signals from the bone marrow microenvironment, complex transcription factor networks and epigenetic regulators. LÄS MER
2. Improving integration efficiency and precision of CRISPR/Cas9-mediated genome editing
Sammanfattning : The clustered regularly interspaced short palindromic repeats (CRISPR) – CRISPR-associated protein 9 (Cas9) system has revolutionized the field of genome engineering, providing a cost-effective and fast tool for targeted gene modifications. Endogenous repair pathways, including error-prone non-homologous end joining and alternative end joining, or precise homology-directed repair (HDR), mend Cas9-induced DNA double-strand breaks. LÄS MER
3. CRISPR/Cas9-based therapies and the role of astrocytes in Alzheimer’s disease and Parkinson’s disease
Sammanfattning : Alzheimer’s disease (AD) and Parkinson’s disease (PD) are the two most common neurodegenerative disorders. Whereas the AD brain features plaques of amyloid-beta (Aβ) and neurofibrillary tangles of tau, the PD brain is characterized by Lewy bodies and Lewy neurites containing α-synuclein (αSyn). LÄS MER
4. Exploring early development and regenerative medicine using CRISPR/Cas9
Sammanfattning : With their intrinsic capacity to self-renew and their potential to differentiate to specialized tissues, human embryonic stem cells (hESCs) have many applications in the fields of cell therapy and developmental biology. By applying clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) genome engineering on selected genes in hESCs, we are able to create cells with desired characteristics. LÄS MER
5. From GWAS to gene : Zebrafish as a model system for large-scale studies of obesity and comorbidities
Sammanfattning : Obesity increases the risk of developing comorbidities such as cardiovascular and metabolic diseases. However, not all obese individuals develop comorbidities, and those that do not are referred to as “metabolically healthy obesity” individuals. LÄS MER