Sökning: "Cell culture monitoring"

Visar resultat 11 - 15 av 38 avhandlingar innehållade orden Cell culture monitoring.

  1. 11. Pulmonary complications after allogeneic hematopoietic stem cell transplantation

    Författare :Ulrica Forslöw; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : The respiratory tract is one of the most common and serious sites for complications in HSCT patients. In this project the incidence, outcome, and risk factors for patients with infectious or non-infectious pulmonary complications were studied. Bronchoscopy and pulmonary function tests (PFTs) were evaluated as diagnostic tools. LÄS MER

  3. 12. Flow cytometric methods for assessment of cell-mediated immune responses

    Författare :Karina Godoy Ramirez; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Flow cytometry; intracellular cytokine staining ICS ; NK cell; CTL; cytotoxicity; HIV-1;

    Sammanfattning : The aim of this thesis was to develop and improve flow cytometry-based methods for assessment of cellmediated immune responses (CMI) induced by natural infection or vaccination. Traditionally, the measurement of anti genspecific cellular immune responses has been based on the use of complex, labourintensive, and less informative bulkresults assays (51Cr-release, 3 3H-thymidin uptake and Limiting dilution assay, LDA). LÄS MER

  4. 13. Analytical tools for monitoring and control of fermentation processes

    Författare :Heléne Sundström; Sven-Olof Enfors; Carl-Fredrik Mandenius; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Escherichia coli; flow cytometry; software sensors; viability; inclusion bodies; biomass; specific growth rate; stress; population heterogeneity; process analytical technology.; Biochemical process engineering; Biokemisk och bioteknisk processteknik;

    Sammanfattning : The overall objective of this work has been to adopt new developments and techniques in the area of measurement, modelling and control of fermentation processes. Flow cytometry and software sensors are techniques which were considered ready for application and the focus was set on developing tools for research aiming at understanding the relationship between measured variables and process quality parameters. LÄS MER

  5. 14. Digital Image Analysis of Cells : Applications in 2D, 3D and Time

    Författare :Amalka Pinidiyaarachchi; Carolina Wählby; Ewert Bengtsson; Arvid Lundervold; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Digital image analysis; microscopy; fluorescent staining; watershed segmentation; sub-cellular localization; point-like signals; wavelets; cell confluence; cytology; color spaces.; Image analysis; Bildanalys;

    Sammanfattning : Light microscopes are essential research tools in biology and medicine. Cell and tissue staining methods have improved immensely over the years and microscopes are now equipped with digital image acquisition capabilities. The image data produced require development of specialized analysis methods. LÄS MER

  7. 15. In vitro characterization of nanodrugs at model lipid membranes

    Författare :Rickard Frost; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; in vitro screening; nanomedicine; drug delivery; drug carrier; supported lipid bilayer; QCM-D; nanoparticle; cell membrane;

    Sammanfattning : The use of nano-sized drug carriers to improve the efficiency of drug delivery has become well established during the past decades. New nanoparticle (NP) formulations for the administration of biopharmaceuticals (e.g. proteins and peptides) emerge at an increasing rate and the need for methods to evaluate their properties is expanding. LÄS MER