Sökning: "BPTI"

Hittade 5 avhandlingar innehållade ordet BPTI.

1. 1. Magnetic relaxation studies of self-associating and membrane proteins

   Författare :Michael Gottschalk; Lund University Bioimaging Center; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; beta-lactoglobulin A; Biokemisk teknik; Biochemical technology; HEWL; BPTI; bacteriorhodopsin; protein self-association; membrane proteins; NMR; magnetic relaxation dispersion;

   Sammanfattning : Magnetic relaxation dispersion measurements have been performed on aqueous protein solutions to study the dynamics of waters in the proton transport channel of bacteriorhodopsin and the self-association of the proteins BPTI, lysozyme and beta-lactoglobulin. The measurements have focused on the bulk relaxation rates, R1 and R2, of three water nuclei 1H, 2H and 17O that are coupled to the protein environments by exchange and therefore report on properties such as the protein tumbling and dynamics of internal waters. LÄS MER

3. 2. Water and protein solutions studied by field-dependent magnetic relaxation

   Författare :Kristofer Modig; Biofysikalisk kemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; relaxation; protein solvation; protein dynamics; protein denaturation; oxytocin; magnetic relaxation dispersion; nuclear magnetic resonance; lipid-binding protein; hydration; beta-lactoglobulin; BPTI; shielding anisotropy; water; Molecular biophysics; Molekylär biofysik;

   Sammanfattning : In the work presented, nuclear magnetic resonance (NMR) relaxation is used to study wide range of systems. The thesis concerns solvent interactions studied with relaxation techniques that involve measurements at many fields, which allows the separation of individual relaxation mechanisms. LÄS MER

4. 3. Water and Protein Dynamics in Biological Systems Studied by Magnetic Relaxation Dispersion

   Författare :Erik Persson Sunde; Biofysikalisk kemi; []
   Nyckelord :protein dynamics; ubiquitin; BPTI; Escherichia coli; Haloarcula marismortui; nuclear magnetic resonance; protein hydration; magnetic relaxation dispersion; water dynamics; cell water;

   Sammanfattning : The results presented in this thesis demonstrate that the magnetic relaxation dispersion (MRD) technique can provide information of relevance to protein biophysics, magnetic resonance imaging and cell biology. By immobilizing proteins with covalent cross-links, intermittent protein dynamics on the previously inaccessible ns-µs time scale could be probed with MRD via the exchange of water molecules between internal cavities and the surrounding bulk solvent phase. LÄS MER

5. 4. Nuclear Magnetic Relaxation Dispersion and Pulsed-Gradient Spin Echo Studies of Biomolecular Solutions and Amphiphilic Liquid Crystals

   Författare :Haukur Jóhannesson; Biofysikalisk kemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; stretched dispersion; DNA hydration; solvent dynamics; NMRD; Random flight simulation; PGSE; Diffusion; Nematic phase; NMR; Amphiphilic liquid crystals; Physical chemistry; Fysikalisk kemi;

   Sammanfattning : Biomolecular solutions and amphiphilic liquid crystals are studied by Nuclear Magnetic Resonance Dispersion (NMRD) and Pulsed-Gradient Spin Echo (PGSE) respectively. A random-flight simulation is performed to obtain the obstructing factors for self-diffusion of small molecules in macrofluids. LÄS MER

7. 5. Protein-water interactions studied by molecular dynamics simulations

   Författare :Filip Persson; Biofysikalisk kemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MD simulations; NMR nuclear magnetic resonance ; MRD; water dynamics; protein dynamics; protein hydration; Amide hydrogen exchange; Internal water; compressibility;

   Sammanfattning : Most proteins have evolved to function optimally in aqueous environments, and the interactions between protein and water therefore play a fundamental role in the stability, dynamics, and function of proteins. Although we understand many details of water, we understand much less about the protein-water interface. LÄS MER