Sökning: "protein denaturation"

Visar resultat 1 - 5 av 55 avhandlingar innehållade orden protein denaturation.

  1. 1. Perplexing Protein Puzzles

    Författare :Stina Lindman; Biofysikalisk kemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; calbindin D9k; split-GFP; protein G B1; pKa-values; protein reconstitution; Electrostatic interactions; protein stability; protein denaturation; heteronuclear NMR; pH titrations;

    Sammanfattning : Protein structure and stability are inherent in the amino acid sequence and governed by non-covalent interactions. The cooperation between forces is, however, perplexing and not well understood. In order to elucidate and predict protein folding and stability, detailed studies of non-covalent interactions are required. LÄS MER

  3. 2. Water and protein solutions studied by field-dependent magnetic relaxation

    Författare :Kristofer Modig; Biofysikalisk kemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; relaxation; protein solvation; protein dynamics; protein denaturation; oxytocin; magnetic relaxation dispersion; nuclear magnetic resonance; lipid-binding protein; hydration; beta-lactoglobulin; BPTI; shielding anisotropy; water; Molecular biophysics; Molekylär biofysik;

    Sammanfattning : In the work presented, nuclear magnetic resonance (NMR) relaxation is used to study wide range of systems. The thesis concerns solvent interactions studied with relaxation techniques that involve measurements at many fields, which allows the separation of individual relaxation mechanisms. LÄS MER

  4. 3. On the mechanism of Urea-induced protein denaturation

    Författare :Matteus Lindgren; Per-Olof Westlund; Arnold Maliniak; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Chemical denaturation; Protein unfolding; urea; MD simulations; NMR spectroscopy; Biophysical chemistry; Biofysikalisk kemi; fysikalisk kemi; Physical Chemistry;

    Sammanfattning : It is well known that folded proteins in water are destabilized by the addition of urea. When a protein loses its ability to perform its biological activity due to a change in its structure, it is said to denaturate. LÄS MER

  5. 4. Protein Misfolding in Human Diseases

    Författare :Karin Almstedt; Per Hammarström; Daniel Otzen; Linköpings universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Misfolding; carbonic anhydrase; prion protein; protein stability; Biochemistry; Biokemi;

    Sammanfattning : There are several diseases well known that are due to aberrant protein folding. These types of diseases can be divided into three main categories:Loss-of-function diseasesGain-of-toxic-function diseasesInfectious misfolding diseases Most loss-of-function diseases are caused by aberrant folding of important proteins. LÄS MER

  7. 5. The Interaction Between the Chaperonin GroEL and Human Carbonic Anhydrase II : Protein Folding and Stability Studies

    Författare :Malin Persson; Linköpings universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; carbonic anhydrase; protein folding; protein stability; chaperone; GroEL; folding mechanism; site-directed spinlabeling; electron paramagnetic resonance EPR ;

    Sammanfattning : The presence of GroEL/ES during the refolding of human carbonic anhydrase II was found to increase the yield of active enzyme from approx. 70 to approx. 100%. This chaperone action on the enzyme could be obtained by adding GroEL alone, and the time course in that case was only moderately slower than the spontaneous process. LÄS MER