Sökning: "single-cell analysis"

Visar resultat 16 - 20 av 216 avhandlingar innehållade orden single-cell analysis.

1. 16. Development of quantitative optical microscopy methods for single cell analysis

   Författare :Katarina Logg; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein localization; quantitative optical microscopy; live cell imaging; image analysis; cellular stress;

   Sammanfattning : Optical microscopy has brought powerful methods to biological research, in particular the possibility to assay dynamic molecular processes within living individual cells. This thesis deals with various aspects of fluorescence and Raman microscopy, such as the possibility of avoiding cellular damage or stress caused by illumination, how to manipulate single cells for spectroscopic analysis and how to quantify images automatically. LÄS MER

3. 17. Genetic Sequence Analysis by Microarray Technology

   Författare :Emilie Hultin; Joakim Lundeberg; Afshin Ahmadian; Marie Allen; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Genotyping; single nucleotide polymorphism SNP ; protease-mediated allele-specific extension PrASE ; microarray; tag-array; competitive hybridization; human papillomavirus HPV ; single cell; loss of alleles; differentiation; epidermis.; Bioengineering; Bioteknik;

   Sammanfattning : Developments within the field of genetic analysis have during the last decade become enormous. Advances in DNA sequencing technology have increased throughput from a thousand bases to over a billion bases in a day and decreased the cost thousandfold per base. LÄS MER

4. 18. Evolving the Methodology for Detection of Primary DNA Damage : Development, adaptation and assessment of the single cell gel electrophoresis (comet) assay

   Författare :Erik Bivehed; Björn Hellman; Hanna Karlsson; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Comet assay; DNA damage; DNA strand breaks; Flash-comet; Genotoxicity; In vitro; Single cell gel electrophoresis; Polymerase assisted DNA damage assay PADDA ; TK6 cells; UCDAS; U937 cells; Toxikologi; Toxicology; Farmaceutisk vetenskap; Pharmaceutical Science;

   Sammanfattning : Deoxyribonucleic acid (DNA) is one of the most important molecules in nature. It is the fundamental carrier of evolutionary information and constitutes the genetic blueprint of all living organisms. Being the sole source of information, it is vital for the cell to transmit the correct genetic information from generation to generation. LÄS MER

5. 19. Massively parallel analysis of cells and nucleic acids

   Författare :Julia Sandberg; Joakim Lundeberg; Nader Pourmand; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Massively parallel sequencing; 454; Illumina; multiplex amplification; whole genome amplification; single cell; polyguanine; flow-cytometry;

   Sammanfattning : Recent proceedings in biotechnology have enabled completely new avenues in life science research to be explored. By allowing increased parallelization an ever-increasing complexity of cell samples or experiments can be investigated in shorter time and at a lower cost. LÄS MER

7. 20. From single-cell transcriptomics to single-molecule counting

   Författare :Saiful Islam; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : RNA-sequencing (RNA-seq) technology has been progressing so fast in the last few years and made it possible to perform transcriptome analysis at single-cell level that was even unimaginable a few years before. Nowadays, the importance of gene expression analysis at the single-cell level is increasingly appreciated for the study of complex heterogeneous tissue. LÄS MER