Avancerad sökning

Hittade 2 avhandlingar som matchar ovanstående sökkriterier.

1. 1. Synthesis and protein curing abilities of membrane glycolipids

   Författare :Malin Wikström; Åke Wieslander; Anthony Lee; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; membrane; lipids; glycolipids; nonbilayer-prone; Biochemistry; Biokemi;

   Sammanfattning : There are many types of membrane lipids throughout Nature. Still little is known about synthesizing pathways and how different lipids affect the embedded membrane proteins. The most common lipids are glycolipids since they dominate plant green tissue. Glycolipids also exist in mammal cells as well as in most Gram-positive bacteria. LÄS MER

3. 2. Intracellular vesicles induced by monotopic membrane protein in Escherichia coli

   Författare :Hanna M. Eriksson; Åke Wieslander; Meta Kuehn; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Membrane protein; intracellular vesicles; Escherichia coli; glycosyltransferase; overexpression; optimization; detergent; screening; lipid composition; Biochemistry; Biokemi; Biochemistry; biokemi;

   Sammanfattning : The monotopic membrane protein alMGS, a glycosyltransferase catalyzing glucolipid synthesis in Acholeplasma laidlawii, was overexpressed in Escherichia coli. Optimization of basic growth parameters was performed, and a novel method for detergent and buffer screening using a small size-exclusion chromatography was developed. LÄS MER