Sökning: "Meta Kuehn"

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1. 1. Intracellular vesicles induced by monotopic membrane protein in Escherichia coli

   Författare :Hanna M. Eriksson; Åke Wieslander; Meta Kuehn; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Membrane protein; intracellular vesicles; Escherichia coli; glycosyltransferase; overexpression; optimization; detergent; screening; lipid composition; Biochemistry; Biokemi; Biochemistry; biokemi;

   Sammanfattning : The monotopic membrane protein alMGS, a glycosyltransferase catalyzing glucolipid synthesis in Acholeplasma laidlawii, was overexpressed in Escherichia coli. Optimization of basic growth parameters was performed, and a novel method for detergent and buffer screening using a small size-exclusion chromatography was developed. LÄS MER