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Visar resultat 1 - 5 av 65 avhandlingar som matchar ovanstående sökkriterier.

1. 1. A Solution Exchange Platform for Exposure of Cells to Chemical Waves

   Författare :Jessica Olofsson; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; waveform synthesis; solution exchange; cell signalling; patch clamp; receptor; high through-put; microfluidics; microfabrication; channel; chemical oscillations; ion channel; poly dimethylsiloxane ; drug discovery;

   Sammanfattning : This thesis presents the development, characterization and suggested applications of a platform for controlling the chemical environment around a cell or a cell constituent. The platform is unique in its capability to combine rapid solution switching with precise timing and access to a manifold of solution environments. LÄS MER

3. 2. Patch-clamp studies of the GABAA receptor using microfluidic methods

   Författare :Maria Millingen; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; ion channel; microfluidics; GABAA receptor;

   Sammanfattning : Ion channels are membrane proteins that passively transport ions. When activated, conformational changes within the protein lead to opening of a channel pore, and the flow of ions causes a change in the membrane potential. This is the fundamental process behind the generation and transduction of nerve impulses. LÄS MER

4. 3. Elucidating the Gating Mechanism of Cys-Loop Receptors

   Författare :Özge Yoluk; Erik Lindahl; Philip Biggin; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; ion channel; gating; simulation; molecular dynamics; receptor; cys-loop; modelling; Teoretisk kemi och biologi; Theoretical Chemistry and Biology; Biological Physics; Biologisk fysik;

   Sammanfattning : Cys-loop receptors are membrane proteins that are key players for the fast synaptic neurotransmission. Their ion transport initiates new nerve signals after activation by small agonist molecules, but this function is also highly sensitive to allosteric modulation by a number of compounds such as anesthetics, alcohol or anti-parasitic agents. LÄS MER

5. 4. Studies of ligand- and temperature-gated ion channels using microfluidic methods

   Författare :Maria Millingen; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; solution exchange; desensitization; temperature-gated; ion channel; temperature control; ligand-gated; TRPV1; GABAA receptor; microfluidics;

   Sammanfattning : This thesis describes the studies of the GABAA receptor, a ligand-gated ion channel, and the ligand- and temperature-gated ion channel TRPV1. The desensitization behavior of both of these proteins has been studied for different conditions, using a microfluidic device for superfusion. LÄS MER

7. 5. Applying the Pump and Leak Hypothesis. An experimental and theoretical investigation of the principles of ion regulation and excitability in the crustacean stretch receptor neuron

   Författare :Sten Theander; Människan i rörelse: hälsa och rehabilitering; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Neurology; neuropsychology; lobster; stretch receptor neuron; leak current; Q-current; K-Cl cotransporter; A-current; mathematical model; excitability; GABA channel; neurophysiology; ion regulation; neuropsykologi; Neurologi; neurofysiologi;

   Sammanfattning : An invertebrate nerve cell preparation, the lobster stretch receptor neuron, was investigated with respect to maintenance of its resting voltage, intracellular ion concentrations and excitability using voltage clamp technique and ion-selective microelectrodes. It was found that the resting voltage (-65 mV) of microelectrode impaled cells is caused by (a) a homogenous population of K+ channels which we denoted as leak channels (b) an electrogenic and voltage-dependent Na-K pump current and (c) an inward microelectrode-induced "impalement" current carried mainly by Na+. LÄS MER