Sökning: "Sophia Hober"

Visar resultat 6 - 10 av 19 avhandlingar innehållade orden Sophia Hober.

  1. 6. Strategies for facilitated protein recovery after recombinant production in Escherichia coli

    Författare :My Hedhammar; Sophia Hober; Alois Jungbauer; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ion-exchange chromatography; protein A; Z; Zbasic; Zacid; fusion protein; proteolytic cleavage; immobilised protease; flow cytometry; inclusion bodies; solid-phase refolding; Molecular biology; Molekylärbiologi;

    Sammanfattning : The successful genomic era has resulted in a great demand for efficient production and purification of proteins. The main objective of the work described in this thesis was to develop methods to facilitate recovery of target proteins after recombinant production in Escherichia coli. LÄS MER

  3. 7. Engineering alternative scaffold proteins for conditional targeting

    Författare :Malin Jönsson; Sophia Hober; Mark Howarth; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Protein Engineering; Conditional targeting; Calcium; Biotechnology; Bioteknologi;

    Sammanfattning : Engineering naturally occurring proteins enables us to customize affinity domains according to our specific needs, tailoring them to become an important tool in a wide array of applications limited merely by our creativity. One of nature’s ways to regulate protein activity is by creating a functional change through alteration of a protein’s tertiary structure upon interaction with metal ions. LÄS MER

  4. 8. Engineering of small IgG binding domains for antibody labelling and purification

    Författare :Sara Kanje; Sophia Hober; Cecília Roque; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody; labelling; purification; Protein G; Protein A; protein engineering; protein design; combinatorial selection; Bioteknologi; Biotechnology;

    Sammanfattning : In protein engineering, rational design and selection from combinatorial libraries are methods used to develop proteins with new or improved features. A very important protein for the biological sciences is the antibody that is used as a detecting agent in numerous laboratory assays. LÄS MER

  5. 9. Development of a covalent site-specific antibody labeling strategy by the use of photoactivable Z domains

    Författare :Anna Konrad; Sophia Hober; Hjalmar Brismar; Patrik Samuelsson; KTH; []
    Nyckelord :antibody labeling; site-specific; covalent; SPPS; Z domain; BPA; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : The joining of two molecular functions or the strategy of adding functions to proteins has been tremendously important for the development of proteins as tools in research and clinic. Depending on the intended application, there are a wide variety of functions that can be added to a proteins. LÄS MER

  7. 10. Generation and characterization of antibodies for proteomics research

    Författare :Karin Larsson; Sophia Hober; John McCafferty; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody; antibody validation; immunohistochemistry; immunofluorescence; tissue microarray; Western blot; epitope mapping; antigen design; Bioengineering; Bioteknik;

    Sammanfattning : Specific antibodies are invaluable tools for proteomics research. The availability of thoroughly validated antibodies will help to improve our understanding of protein expression, localization and function; fundamental processes and features of all living organisms. LÄS MER