Sökning: "Protein-DNA interactions"

Visar resultat 6 - 10 av 19 avhandlingar innehållade orden Protein-DNA interactions.

  1. 6. Application of proximity Ligation for Detection of Proteins, Biomolecular Interactions, and Single Copies of Pathogens

    Författare :Sigrun Margret Gustafsdottir; Ulf Landegren; Per-Åke Nygren; Uppsala universitet; []
    Nyckelord :Molecular medicine; Proximity ligation; Cytokines; Microbial pathogens; Protein-DNA interactions; Drug screening; Molekylärmedicin;

    Sammanfattning : Proximity ligation is a recently established technique that can provide answers to questions about the concentration, localization, interactions, modifications and functions of proteins. The method enables sensitive protein measurements with a detection limit in the low femtomolar range in complex biological samples. LÄS MER

  3. 7. Modelling biomolecular interactions

    Författare :Johan Bredenberg; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Computational chemistry; molecular dynamics; nuclear receptors; force field parameterization; zinc fingers; DNA; proteins.;

    Sammanfattning : Computational approaches for understanding and aiding in molecular biology has increased in significance over the last decades, where a wealth of biochemical experiments have provided a solid ground for developing computer models that can be used to predict unresolved issues within biology. Molecular dynamics (MD) is one of these methods, based on classical laws, and suitable for handling large macromolecules in their natural environment, water. LÄS MER

  4. 8. Molecular Metamorphosis in Transcriptional Regulation

    Författare :Malin Lüking; Johan Elf; Victor Muñoz; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Protein-DNA interactions; Facilitated diffusion; Binding Kinetics; Molecular Simulations; Fluorescence Microscopy; Biology with specialization in Structural Biology; Biologi med inriktning mot strukturbiologi; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Sammanfattning : The foundation of all life is the interaction of molecules. Molecular interaction occurs in the tightly packed cytoplasm. In this crowded environment, the molecules need to be able to establish stable interactions with specific interaction partners, and with specific ones only. LÄS MER

  5. 9. Visualizing Interacting Biomolecules In Situ

    Författare :Irene Weibrecht; Ola Söderberg; Ulf Landegren; Banafshe Larijani; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation; in situ PLA; padlock probe; rolling circle amplification; flow cytometry; in situ; single cell; single molecule; protein interaction; protein-DNA interaction; posttranslational modification; Molecular medicine; Molekylär medicin; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Intra- and intercellular information is communicated by posttranslational modifications (PTMs) and protein-protein interactions, transducing information over cell membranes and to the nucleus. A cells capability to respond to stimuli by several highly complex and dynamic signaling networks provides the basis for rapid responses and is fundamental for the cellular collaborations required in a multicellular organism. LÄS MER

  7. 10. Functional and structural properties of eukaryotic DNA polymerase epsilon

    Författare :Olga Chilkova; Erik Johansson; Per Elias; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; eukaryotic DNA replication; DNA polymerase epsilon; protein-DNA interaction; Biochemistry; Biokemi;

    Sammanfattning : In eukaryotes there are three DNA polymerases which are essential for the replication of chromosomal DNA: DNA polymerase alpha (Pol alpha), DNA polymerase delta (Pol delta) and DNA polymerase epsilon (Pol epsilon). In vitro studies of viral DNA replication showed that Pol alpha and Pol delta are sufficient for DNA replication on both leading and lagging DNA strands, thus leaving the function of Pol epsilon unknown. LÄS MER