Sökning: "HepG2"

Visar resultat 1 - 5 av 47 avhandlingar innehållade ordet HepG2.

  1. 1. Development and Applications of Microfluidic Devices for Liver-on-a-Chip Studies

    Författare :Philip Dalsbecker; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Microfluidics; Microphysiological system; Liver-on-a-Chip; Organ-on-a-chip; Hepatocytes; Induced pluripotent stem cells; iPSC; HepG2; LX-2; on-chip differentiation; PDMS; Microfabrication; Shear stress;

    Sammanfattning : Modeling the human physiology in vitro is a challenging task, yet one of importance for the development of drugs and the study of diseases. To better address the complexities of human organs, microfluidic systems called organs-on-chips are being developed to emulate organ-specific environments for cell culture. LÄS MER

  3. 2. Genome-Wide Studies of Transcriptional Regulation in Mammalian Cells

    Författare :Ola Wallerman; Claes Wadelius; Jussi Taipale; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ChIP; ChIP-chip; ChIP-seq; transcription factors; motif discovery; nucleosome positioning; HepG2; genome-wide; RNA-seq; Medical genetics; Medicinsk genetik; Medical Genetics; Medicinsk genetik;

    Sammanfattning : The key to the complexity of higher organisms lies not in the number of protein coding genes they carry, but rather in the intrinsic complexity of the gene regulatory networks. The major effectors of transcriptional regulation are proteins called transcription factors, and in this thesis four papers describing genome-wide studies of seven such factors are presented, together with studies on components of the chromatin and transcriptome. LÄS MER

  4. 3. Single-Proton Irradiation of Living Cells - Development of New Tools for Low-Dose Radiation Research

    Författare :Natalia Arteaga; Kärnfysik; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; lithography; UVL; EBL; SeACell; human hepatoma cells HepG2 ; reactive oxygen species ROS ; bystander effect; SU-8; cell recognition; cell irradiation; Single-Ion Hit Facility; low-dose; Fysicumarkivet A:2010:Arteaga Marrero;

    Sammanfattning : A Single-Ion Hit Facility (SIHF) consists of a custom-build facility based in particle accelerators which offers irradiation controlling the number of delivered particles with a precise targeting localization. The irradiation spot can be confined down to the nanometre scale allowing the irradiation of subcellular compartments with a single particle. LÄS MER

  5. 4. Fighting cardiometabolic disease : validation of new experimental models and therapeutic targets

    Författare :Mirko Enea Minniti; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : The prevalence of cardiometabolic diseases (CMD) such as atherosclerotic cardiovascular diseases, type 2 diabetes mellitus and nonalcoholic fatty liver disease (NAFLD), has grown dramatically during the last decades. Hence, massive research efforts are allocated to identify the pathophysiological mechanisms and new therapeutic targets for these morbidities. LÄS MER

  7. 5. Regulation of IL-6-signaling and inflammatory response – Role of insulin, FOXO1 and PKCδ

    Författare :Emelie Wallerstedt; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Type 2-Diabetes; inflammation; obesity; IL-6; STAT3; PKCδ; FOXO1;

    Sammanfattning : The aim of this thesis was to investigate if and how insulin antagonizes interleukin-6 (IL-6)-signaling and action using both 3T3-L1 mouse adipocytes (Paper I) and HepG2 human hepatocytes (Paper III). We also investigated the importance of PKCδ in 3T3-L1 adipocytes and mouse embryonic fibroblasts (MEFs) lacking protein kinase C-δ (PKCδ-/-) (Paper II). LÄS MER