Sökning: "DNA Sample Preparation"

Visar resultat 1 - 5 av 34 avhandlingar innehållade orden DNA Sample Preparation.

  1. 1. Microfluidic Preparation and Transport of Long DNA using Pillar Arrays

    Författare :Oskar Ström; NanoLund: Centre for Nanoscience; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Microfluidics; Deterministic Lateral Displacement; DNA Separation; Elastic Turbulence; Micro-pillar arrays; DNA Sample Preparation; Lab-on-a chip; elastic waves; Sample Preparation; DNA Isolation; bumper array; Fysicumarkivet A:2023:Ström;

    Sammanfattning : This thesis presents a set of microfluidic tools and experimental studies for preparing (> 20 kbp) for genetic analysisas well as the transport of high-concentration, long-DNA solutions in pillar arrays.Long-DNA sample preparation with conventional gel-based techniques is slow (tens of hours to days) and laborious. LÄS MER

  3. 2. On-chip Ultrasonic Sample Preparation

    Författare :Ida Sadat Iranmanesh; Martin Viklund; Johan Nilsson; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Ultrasound; Sample preparation; Particle manipulation; Fysik; Physics; Biological Physics; Biologisk fysik;

    Sammanfattning : Acoustofluidics has become a well-established technology in the lab-on-a-chip scientific community. The technology involves primarily the manipulation of fluids and/or particles in microfluidic systems. LÄS MER

  4. 3. Library Preparation for High Throughput DNA Sequencing

    Författare :Anders Jemt; Joakim Lundeberg; Petri Auvinen; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA; RNA; sequencing; massively parallel sequencing; library preparation; automation; genome; transcriptome; Bioteknologi; Biotechnology;

    Sammanfattning : Order 3 billion base pairs of DNA in the correct order and you get the blueprint of a human, the genome. Before the introduction of massively parallel sequencing a little more than a decade ago it would cost around $10 million to get this blueprint. LÄS MER

  5. 4. Immuno-affinity interactions in sample preparation and analysis

    Författare :Madalina Tudorache; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; immuno supported liquid membrane ISLM extraction; sample preparation; immunoassay; immuno-affinity interaction; antibody; Analytical chemistry; Analytisk kemi;

    Sammanfattning : Immuno-affinity interactions (antibody-antigen) belong, together with enzyme-inhibitor/substrate/coenzyme; oligosaccharide-lectin; DNA/RNA-protein interactions etc, to the class of bio-recognition. Immuno-affinity is widely used for analysis in immunoassays with numerous applications in a variety fields (i.e. LÄS MER

  7. 5. Methods to Prepare DNA for Efficient Massive Sequencing

    Författare :Sverker Lundin; Joakim Lundeberg; Harold Swerdlow; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; DNA; Massive sequencing; Next Generation Sequencing; Library Preparation; Barcoding; Multiplexing;

    Sammanfattning : Massive sequencing has transformed the field of genome biology due to the continuous introduction and evolution of new methods. In recent years, the technologies available to read through genomes have undergone an unprecedented rate of development in terms of cost-reduction. LÄS MER