Sökning: "Library Preparation"

Visar resultat 1 - 5 av 41 avhandlingar innehållade orden Library Preparation.

1. 1. Library Preparation for High Throughput DNA Sequencing

   Författare :Anders Jemt; Joakim Lundeberg; Petri Auvinen; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA; RNA; sequencing; massively parallel sequencing; library preparation; automation; genome; transcriptome; Bioteknologi; Biotechnology;

   Sammanfattning : Order 3 billion base pairs of DNA in the correct order and you get the blueprint of a human, the genome. Before the introduction of massively parallel sequencing a little more than a decade ago it would cost around $10 million to get this blueprint. LÄS MER

3. 2. Methods to Prepare DNA for Efficient Massive Sequencing

   Författare :Sverker Lundin; Joakim Lundeberg; Harold Swerdlow; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; DNA; Massive sequencing; Next Generation Sequencing; Library Preparation; Barcoding; Multiplexing;

   Sammanfattning : Massive sequencing has transformed the field of genome biology due to the continuous introduction and evolution of new methods. In recent years, the technologies available to read through genomes have undergone an unprecedented rate of development in terms of cost-reduction. LÄS MER

4. 3. Advances in DNA Detection

   Författare :Yajing Song; Olof Emanuelsson; Jonas Tegenfeldt; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; DNA detection; active filter paper; visual detection; throughput; fluorescence; superparamagnetic beads; rare DNA; Q-PCR; chemical fragmentation; DNA library preparation; massively parallel sequencing; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : DNA detection technologies have an increasing importance in our everyday lives, with applications ranging from microbial diagnostics to forensic analysis, food safety evaluation, and environmental monitoring. Currently, as the associated costs decrease, DNA diagnostic techniques are routinely used in research laboratories, in clinical and forensic practice. LÄS MER

5. 4. Simplified Routines for Sample Preparation and Analysis of Chemical Warfare Agent Degradation Products

   Författare :Raja Subramaniam; Calle Nilsson; Paula Vanninen; Umeå universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Nerve agents; Alkyl alkylphosphonic acids; Solid phase derivatization; Fluorinated diazomethane reagent; High-energy collision induced dissociation; CID MS MS library; analytisk kemi; Analytical Chemistry;

   Sammanfattning : The thesis describes the development of new and improved methods for analyzing degradation markers from organophosphorus Chemical Warfare Agents (CWAs). Paper I and II describes an innovative and significantly improved method for the enrichment, derivatization (trimethysilylation) and GC-MS analysis of a broad range of organophosphorus CWAs degradation markers, namely the alkylphosphonic acids and a zwitterionic compound. LÄS MER

7. 5. Managing chemotherapy risks : Learning from medication errors and developing a national knowledge source for chemotherapy regimens

   Författare :Ann-Sofie Fyhr; Ergonomi och aerosolteknologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; SAMHÄLLSVETENSKAP; SOCIAL SCIENCES; Chemotherapy; Chemotherapy regimens; Medication errors; Patient safety; e-library; Standardisation; User evaluation; Usability; Chemotherapy; Chemotherapy regimens; Medication errors; Patient safety; e-library; Standardisation; User evaluation; Usability;

   Sammanfattning : The basics in cancer treatment are surgery, radiation therapy, and treatment with cancer drugs, often combined. Chemotherapy regimens that define the drugs used, the dosage, the frequency and duration of drug administration, have been developed and used for different cancer diagnoses. LÄS MER