Sökning: "single-cell whole genome amplification"

Hittade 5 avhandlingar innehållade orden single-cell whole genome amplification.

  1. 1. Technologies for Single Cell Genome Analysis

    Författare :Erik Borgström; Afshin Ahmadian; Sten Linnarsson; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA; sequencing; single molecule; single cell; whole genome amplification; exome sequencing; emulsions; barcoding; phasin; Biotechnology; Bioteknologi;

    Sammanfattning : During the last decade high throughput DNA sequencing of single cells has evolved from an idea to one of the most high profile fields of research. Much of this development has been possible due to the dramatic reduction in costs for massively parallel sequencing. LÄS MER

  3. 2. Massively parallel analysis of cells and nucleic acids

    Författare :Julia Sandberg; Joakim Lundeberg; Nader Pourmand; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Massively parallel sequencing; 454; Illumina; multiplex amplification; whole genome amplification; single cell; polyguanine; flow-cytometry;

    Sammanfattning : Recent proceedings in biotechnology have enabled completely new avenues in life science research to be explored. By allowing increased parallelization an ever-increasing complexity of cell samples or experiments can be investigated in shorter time and at a lower cost. LÄS MER

  4. 3. Single-cell population genetics and dispersal limitation of a bloom-forming microalga

    Författare :Raphael Gollnisch; Enhet akvatisk ekologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Gonyostomum semen; algal blooms; invasive species; population structure; RADseq; single-cell whole genome amplification; multiple displacement amplification; calcium; pH; iron;

    Sammanfattning : Microalgal range expansions are increasing in frequency and magnitude worldwide mostly due to anthropogenic factors. However, they often remain unnoticed until mass development (algal blooms) occurs due to difficulties in detection when population densities are low. LÄS MER

  5. 4. Developing methods for mapping genetic heterogeneity in tumors : from bulk to single-cell resolution

    Författare :Michele Simonetti; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Tumors are complex ecosystems composed of billions of cells that constantly evolve over time. Intra-tumor heterogeneity (ITH) represents the diversity of this complex environment quantifiable both at the genetic and phenotypic level. Recently, next-generation sequencing (NGS) costs have dropped and NGS has become the standard tool for studying ITH. LÄS MER

  7. 5. Interpreting the human transcriptome

    Författare :Beata Werne Solnestam; Joakim Lundeberg; Piero Carninci; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Transcriptome; RNA sequencing; high-throughput sequencing; gene expression profiling; multiplex amplification; Bioteknologi; Biotechnology;

    Sammanfattning : The human body is made of billions of cells and nearly all have the same genome. However, there is a high diversity of cells, resulted from what part of the genome the cells use, i.e. which RNA molecules are expressed. LÄS MER