Sökning: "single-cell DNA sequencing"

Visar resultat 1 - 5 av 35 avhandlingar innehållade orden single-cell DNA sequencing.

  1. 1. Technologies for Single Cell Genome Analysis

    Författare :Erik Borgström; Afshin Ahmadian; Sten Linnarsson; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA; sequencing; single molecule; single cell; whole genome amplification; exome sequencing; emulsions; barcoding; phasin; Biotechnology; Bioteknologi;

    Sammanfattning : During the last decade high throughput DNA sequencing of single cells has evolved from an idea to one of the most high profile fields of research. Much of this development has been possible due to the dramatic reduction in costs for massively parallel sequencing. LÄS MER

  3. 2. Variational methods for phylogeny and single-cell genomics

    Författare :Hazal Koptagel; Jens Lagergren; Alexandre Bouchard-Côté; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Variational inference; single-cell DNA sequencing; cell lineage tree; Bayesian phylogenetic tree inference; Variationsinferens; encellig DNA-sekvensering; cellhärkomstträd; Bayesiansk fylogenetisk trädinferens; Varyasyonel çıkarım; tek hücre DNA dizilimi; hücre soy ağacı; Bayesçi filogenetik ağaç çıkarımı; Datalogi; Computer Science;

    Sammanfattning : The investigation of the evolutionary history of organisms, both at the cellular level and at the species level, is a relevant research topic in computational biology. These investigations lead to a deeper understanding of developmental history, cancer progression, the genetic similarity of species, and more. LÄS MER

  4. 3. Detection and analysis of genetic alterations in normal skin and skin tumours

    Författare :Åsa Sivertsson; KTH; []
    Nyckelord :single cell; DNA sequencing; p53; mutation; UV; BCC; pyrosequencing; malignant melanoma; N-ras;

    Sammanfattning : The investigation of genetic alterations in cancer-relatedgenes is useful for research, prognostic and therapeuticpurposes. However, the genetic heterogeneity that often occursduring tumour progression can make correct analysischallenging. LÄS MER

  5. 4. Novel insights into protist diversity and niche adaptation using single cell transcriptomics

    Författare :Henning Onsbring; Thijs J. G. Ettema; Alastair Simpson; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Protists; microbial eukaryotes; cultivation-independent methods; single-cell RNA sequencing; phylogenomics; Biologi med inriktning mot molekylär evolution; Biology with specialization in Molecular Evolution;

    Sammanfattning : Protists are a polyphyletic group of microbes that represents the vast majority of eukaryotic diversity. Despite this, most sequencing efforts targeting eukaryotes have been focused on animals, fungi and plants. LÄS MER

  7. 5. Exploring human variations by droplet barcoding

    Författare :Pontus Höjer; Afshin Ahmadian; Pelin Sahlén; Lars Feuk; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; droplets; linked-read sequencing; DNA barcoding; proteomics; genomics; single cell; single extracellular vesicle; single exosome; pipelines; Biotechnology; Bioteknologi;

    Sammanfattning : Biological variations are being explored at ever-increasing rates through the rapid advancement of analytical techniques. Techniques like massively parallel sequencing empower scientists to accurately differentiate individuals’ genetic compositions, cellular functionalities, and healthy tissue from diseased. LÄS MER