Sökning: "Sarah Butcher"

Hittade 3 avhandlingar innehållade orden Sarah Butcher.

  1. 1. Towards unbiased 3D reconstruction : in single-particle cryo-electron microscopy

    Författare :Dominika Elmlund; Hans Hebert; Sarah Butcher; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Bioengineering; Bioteknik;

    Sammanfattning : Cryo-electron microscopy of freestanding molecules (single-particles) plays a pivotal role in the difficult and pressing challenge of determining the structures of large macromolecular complexes. Molecular volumes are generated by aligning large sets of randomly oriented two-dimensional (2D) projection images in three dimensions (3D) before reconstruction is performed using tomographic techniques. LÄS MER

  3. 2. Structural Studies of Large dsDNA Viruses using Single Particle Methods

    Författare :Hemanth Kumar Narayana Reddy; Martin Svenda; Sarah Butcher; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; PR772; phage; PRD1; Bacteriophage; coliphage; Melbournevirus; Cedratvirus; lurbo; Pithovirus; CryoEM; Single particle imaging; Coherent; Diffractive; Imaging; Soft X-ray; Microscopy; Microscope; GDVN; High resolution; XFEL; aerosol; Injection; electrospray; gas dynamic virtual nozzle; CDI; CXI; FEL; Chemistry with specialization in Biophysics; Kemi med inriktning mot biofysik;

    Sammanfattning : Structural studies of large biological assemblies pose a unique problem due to their size, complexity and heterogeneity. Conventional methods like x-ray crystallography, NMR, etc. are limited in their ability to address these issues. To overcome some of these limitations, single particle methods were used. LÄS MER

  4. 3. Structural Decorations in Viruses : Unraveling Acquired Functional Structures in Icosahedral RNA Virus Capsids

    Författare :Han Wang; Kenta Okamoto; Sarah Butcher; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Viruses; RNA viruses; capsid; structure; cryo-EM; Molekylär biovetenskap; Molecular Life Sciences;

    Sammanfattning : Viruses have a profound impact worldwide, posing challenges to animal welfare, agriculture, human health, and the ecosystem. This thesis examines the realm of non-enveloped icosahedral double-stranded (ds)RNA and single-stranded (ss)RNA viruses through three studies. LÄS MER