Sökning: "ribosomal binding site"

Visar resultat 6 - 10 av 35 avhandlingar innehållade orden ribosomal binding site.

1. 6. Cellular proteins binding to the HCV 5' and 3' UTRs

   Författare :Karin Spångberg; Ingemar Ernberg; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Hepatitis C virus; RNA-binding proteins; mRNA stability; translation; Biokemi; Biochemistry; Biokemi; Medical Virology; medicinsk virologi;

   Sammanfattning : Hepatitis C virus (HCV) is a worldwide health problem with more than 170 million people infected by the virus. About 80% become chronic carriers, which may result in hepatocellular carcinoma. The lack of an in vitro cell culture system that allows propagation of the virus has hampered the development of a vaccine or anti-viral drugs against HCV. LÄS MER

3. 7. Computational Studies of Protein Synthesis on the Ribosome and Ligand Binding to Riboswitches

   Författare :Christoffer Lind; Johan Åqvist; Alexander MacKerell Jr; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Binding free energy; Ribosome; Codon reading; Translation initiation; Translation termination; Mitochondrial translation; Release factor; Purine riboswitch; Molecular Dynamics; Free Energy Perturbation; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

   Sammanfattning : The ribosome is a macromolecular machine that produces proteins in all kingdoms of life. The proteins, in turn, control the biochemical processes within the cell. It is thus of extreme importance that the machine that makes the proteins works with high precision. LÄS MER

4. 8. From Structure to Function with Binding Free Energy Calculations for Codon Reading, Riboswitches and Lectins

   Författare :Johan Sund; Åqvist Johan; Javier Luque; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; binding free energy; ribosome; codon reading; release factor; mitochondrial translation; purine riboswitch; lectin; molecular dynamics; free energy perturbation; Biology with specialization in Structural Biology; Biologi med inriktning mot strukturbiologi;

   Sammanfattning : Molecular association is part of many important processes in living cells. Computational methods for calculating binding free energies allows for a quantitative examination of biomolecular structures and hypotheses drawn from biochemical experiments. LÄS MER

5. 9. Computer simulations of ribosome reactions

   Författare :Stefan Trobro; Johan Åqvist; Arieh Warshel; Uppsala universitet; []
   Nyckelord :Molecular biology; ribosomal termination; Empirical valence bond; peptidyl transfer; Molekylärbiologi;

   Sammanfattning : Peptide bond formation and translational termination on the ribosome have been simulated by molecular mechanics, free energy perturbation, empirical valence bond (MD/FEP/EVB) and automated docking methods. Recent X-ray crystallographic data is used here to calculate the entire free energy surface for the system complete with substrates, ribosomal groups, solvent molecules and ions. LÄS MER

7. 10. In Vitro Kinetics of Ribosomal Incorporation of Unnatural Amino Acids

   Författare :Jinfan Wang; Anthony Forster; Jason Chin; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; ribosome; protein synthesis; unnatural amino acids; kinetics; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

   Sammanfattning : Ribosomal incorporation of unnatural amino acids (AAs) into peptides or proteins has found broad applications in studying translation mechanism, discovering potential therapeutics, and probing protein structure and function. However, such applications are generally limited by the low incorporation efficiencies of the unnatural AAs. LÄS MER