Sökning: "in situ hybridization"

Visar resultat 26 - 30 av 298 avhandlingar innehållade orden in situ hybridization.

  1. 26. Genotyping RNA and DNA using padlock probes

    Författare :Dan-Oscar Antson; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Padlock probe; enzymatic synthesis; PCR; in situ hybridization; RNA-templated DNA ligation; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

    Sammanfattning : Novel techniques are needed to investigate the genetic variation revealed in the first draft of the human genome sequence. Padlock probes are recently developed reagents, suitable for detecting single-nucleotide variations of DNA and RNA in situ or in solution. LÄS MER

  3. 27. Gene and Protein Profiling of the Preeclamptic Placenta

    Författare :Magnus Centlow; Lund Obstetrik och gynekologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; antigen presentation; APOA1; hemoglobin; immunohistochemistry; in situ hybridization; Microarray; 2D-PAGE; uterine artery bilateral notching;

    Sammanfattning : Aims State-of-the-art methodology was used to screen and profile the placenta, gene and protein expression, for changes related to preeclampsia (PE) and cases with increased resistance in the uterine arteries. Women with increased resistance in the uterine arteries have increased risk of developing PE. LÄS MER

  4. 28. Neuropeptide expression in mouse disease models

    Författare :Margarita Diez; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Alzheimers disease; autoradiographic ligand binding; CCK; degeneration; dynorphin; enkephalin; galanin; immunohistochemistry; in situ hybridization; lesion; magnetic resonance imaging; megencephaly; NPY; prion infection; substance P.;

    Sammanfattning : The role of neuropeptides and the significance of peptidergic mechanisms in neurodegenerative diseases are still relatively unexplored. In the periphery, nerve injury results in dramatic changes in the expression of neuropeptides (and other molecules). LÄS MER

  5. 29. Padlock Probes and Rolling Circle Amplification : New Possibilities for Sensitive Gene Detection

    Författare :Maritha Mendel-Hartvig; Pål Nyrén; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Padlock probes; RCR; in situ hybridization; branched DNA; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

    Sammanfattning : A series of novel methods for detection of known sequence variants in DNA, in particular single nucleotide polymorphism, using padlock probes and rolling circle replication are presented. DNA probes that can be circularized – padlock probes – are ideal for rolling circle replication. LÄS MER

  7. 30. Molecular mechanisms of tumor development in hyperparathyroidism

    Författare :Filip Farnebo; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :parathyroid; hyperparathyroidism; MEN 1; FIHP; loss of heterozygosity; comparative genomic hybridization; mutation analysis; in situ hybridization;

    Sammanfattning : Loss of heterozygosity (LOH) analysis and comparative genomic hybridization (CGH) were used in an attempt to identify recurrent chromosomal alterations in different types of parathyroid tumors. These included familial and sporadic, benign and malignant, as well as primary, irradiation associated and secondary tumors. LÄS MER