Sökning: "functional activity assay"

Visar resultat 1 - 5 av 113 avhandlingar innehållade orden functional activity assay.

1. 1. Functional studies of calcein acetoxymethyl ester in human tumor cells

   Författare :Gunnar Liminga; Uppsala universitet; []
   Nyckelord :Medical sciences; Calcein AM; Calcein; Functional assay; Drug resistance; Cytotoxicity; MEDICIN OCH VÅRD; MEDICINE; MEDICIN; klinisk farmakologi; Clinical Pharmacology;

   Sammanfattning : A fluorometric assay for functional measurement of P-glycoprotein 170 (Pgp) mediated transport in cell lines, using the acetoxymethyl ester of calcein (calcein/AM) as a probe was established. The myeloma cell line RPMI 8226/S and the two Pgp-expressing doxorubicin resistant subcell lines 8226/dox6 (low) and 8226/dox40 (high) were used. LÄS MER

3. 2. Plasminogen Activator Inhibitor 1 in Platelets - Studies of Synthesis, Activity, and Glycosylation Patterns

   Författare :Helén Brogren; Göteborgs universitet; []
   Nyckelord :PAI-1; platelets; plasma; fibrinolysis; synthesis; polymorphism; glycosylation; activity; platelet mRNA;

   Sammanfattning : Plasminogen activator inhibitor 1 (PAI-1) is the main physiological inhibitor of tissue-type plasminogen activator. Thus PAI-1 plays an important role in decreasing the fibrinolytic activity in human blood. PAI-1 is present in high concentrations in platelets and also in low concentrations in plasma, but the source of plasma PAI-1 is not known. LÄS MER

4. 3. Ligands of the Angiotensin II Type 2 Receptor : Exploring structure and function of the AT2R ligand C38

   Författare :Rebecka Isaksson; Mats Larhed; Annette Bayer; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Angiotensin II type 2 receptor; AT2R antagonists; sulfonyl carbamates; bicyclic amides; metabolic stability; functional activity assay; pharmacological profile; medicinal chemistry; structure-activity relationship; Medicinal Chemistry; Läkemedelskemi;

   Sammanfattning : The renin-angiotensin-aldosterone-system (RAAS) control blood-pressure regulation, exerted by the main effector peptide angiotensin II (AngII) binding the angiotensin II type 1 receptor (AT1R). While hypertension is the most known disease caused by over-activity in RAAS, several proteins in the system exhibit protective functions. LÄS MER

5. 4. Understanding and manipulating primary cell walls in plant cell suspension cultures

   Författare :Felicia Leijon; Vincent Bulone; Katja Salomon Johansen; KTH; []
   Nyckelord :Callose synthase; carbohydrate-binding module; cell wall engineering; cellulose microfibril; cellulose nanocrystal; glycosyltransferase; mass spectrometry; plasmodesmata; Populus; primary cell wall; radiometric assay; spectrophotometric assay; Biotechnology; Bioteknologi;

   Sammanfattning : The cell wall is required for many aspects of plant function and development. It is also an accessible and renewable resource utilized both in unrefined forms and as raw material for further development. Increased knowledge regarding cell wall structure and components will contribute to better utilization of plants and the resources they provide. LÄS MER

7. 5. Cell-penetrating peptides, novel synthetic nucleic acids, and regulation of gene function : Reconnaissance for designing functional conjugates

   Författare :Peter Guterstam; Ülo Langel; Stockholms universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell-penetrating peptide; nucleic acids; alternative splicing; mismatch discrimination; Neurochemistry; Neurokemi;

   Sammanfattning : Our genome operates by sending instructions, conveyed by mRNA, for the manufacture of proteins from chromosomal DNA in the nucleus of the cell to the protein synthesizing machinery in the cytoplasm. Alternative splicing is a natural process in which a single gene can encode multiple related proteins. LÄS MER