Sökning: "aldolase"

Visar resultat 1 - 5 av 11 avhandlingar innehållade ordet aldolase.

  1. 1. Aldolases for Enzymatic Carboligation : Directed Evolution and Enzyme Structure-Function Relationship Studies

    Författare :Huan Ma; Mikael Widersten; Wolf-Dieter Fessner; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; aldolase; 2-deoxyribose-5-phosphate aldolase; fructose-6-phosphate aldolase; directed evolution; enzyme dynamics; Biokemi; Biochemistry;

    Sammanfattning : The research summarized in this thesis focuses on directed evolution and enzyme mechanism studies of two aldolases: 2-deoxyribose-5-phosphate aldolase (DERA) and fructose-6-phosphate aldolase (FSA).Aldolases are nature’s own catalysts for one of the most fundamental reactions in organic chemistry: the formation of new carbon-carbon bonds. LÄS MER

  3. 2. Rational redesign of Candida antarctica lipase B

    Författare :Anders Magnusson; Karl Hult; Bernhard Hauer; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Candida antarctica lipase B; rational redesign; secondary alcohols; substrate-assisted catalysis; S-selective; entropy; aldolase; stereospecificity pocket; oxyanion hole.; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : This thesis describes the use of rational redesign to modify the properties of the enzyme Candida antarctica lipase B. Through carefully selected single-point mutations, we were able to introduce substrate-assisted catalysis and to alter the reaction specificity. LÄS MER

  4. 3. Crystallographic studies of transaldolase : implications for the enzymatic mechanism and the evolution of class I aldolases

    Författare :Jia Jia; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :transaldolase; aldolase; Schiff-base intermediate; protein crystallography; site directed mutagenesis; catalytic mechanism; circular permutation.;

    Sammanfattning : Transaldolase catalyzes the reversible transfer of a dihydroxyacetone moiety from a ketose donor to an aldose acceptor by forming a Schiff-base intermediate between a Iysine residue and dihydroxyacetone. It belongs to the class I aldolase family. LÄS MER

  5. 4. Sorting nexin 9 in clathrin-mediated endocytosis

    Författare :Richard Lundmark; Sven Carlsson; Elizabeth Smythe; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; sorting nexin; dynamin; clathrin; endocytosis; human; adaptor protein complexes; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi; medicinsk biokemi; Medical Biochemistry;

    Sammanfattning : Clathrin-mediated endocytosis is a process by which cells can internalise diverse molecules such as nutrients, antigens and signalling-surface receptors. The creation of clathrin-coated vesicles demands interplay between the plasma membrane lipids, cargo molecules and the proteins that build up the coat. LÄS MER

  7. 5. The transaldolase family : structure, function and evolution

    Författare :Stina Thorell; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : The transaldolase gene family includes representatives from all kingdoms of life and the encoded enzymes are important for carbohydrate metabolism. The family can be divided into two subfamilies; Classical transaldolases and the MipB/TalC proteins. LÄS MER