Sökning: "rational redesign"
Visar resultat 1 - 5 av 7 avhandlingar innehållade orden rational redesign.
1. Rational redesign of Candida antarctica lipase B
Sammanfattning : This thesis describes the use of rational redesign to modify the properties of the enzyme Candida antarctica lipase B. Through carefully selected single-point mutations, we were able to introduce substrate-assisted catalysis and to alter the reaction specificity. LÄS MER
2. Modulating Enzyme Functions by Semi-Rational Redesign and Chemical Modifications : A Study on Mu-class Glutathione Transferases
Sammanfattning : Today, enzymes are extensively used for many industrial applications, this includes bulk and fine-chemical synthesis, pharmaceuticals and consumer products. Though Nature has perfected enzymes for many millions of years, they seldom reach industrial performance targets. LÄS MER
3. Probing the Alpha Class Glutathione Transferase Structure. Combinatorial Subunit Studies and Rational Redesign
Sammanfattning : The present work concerns two Alpha class enzymes, GST A1-1 and GST A4-4, of the glutathione transferase (GST) family, which are detoxication enzymes catalyzing the conjugation of the tripeptide glutathione with various electrophiles. The GST A1-1 structure was explored for protein engineering purposes and tailored for increased activity toward alkenals. LÄS MER
4. Mutational Analysis and Redesign of Alpha-class Glutathione Transferases for Enhanced Azathioprine Activity
Sammanfattning : Glutathione transferase (GST) A2-2 is the human enzyme most efficient in catalyzing azathioprine activation. Structure-function relationships were sought explaining the higher catalytic efficiency compared to other alpha class GSTs. LÄS MER
5. Exploring the Functional Plasticity of Human Glutathione Transferases : Allelic Variants, Novel Isoenzyme and Enzyme Redesign
Sammanfattning : Glutathione transferases (GSTs) make up a superfamily that is involved in the cellular defense against various reactive compounds by catalyzing the conjugation of glutathione to electrophilic centra. Members of this family have also been implicated in different facets of biological signaling. LÄS MER