Sökning: "Sulfhydryl"
Visar resultat 1 - 5 av 20 avhandlingar innehållade ordet Sulfhydryl.
1. Quantitation, Purification and Reconstitution of the Red Blood Cell Glucose Transporter GLUT1
Sammanfattning : The human glucose transporter GLUT1 facilitates glucose to be accumulated on the other side of the cell membrane. The functional state of GLUT1 is uncertain due to diversity of the reports. In this thesis, the activity of red blood cell GLUT1 was extensively studied to further characterize this protein. LÄS MER
2. Microsomal glutathione transferase
Sammanfattning : A distinct glutathione transferase which can be activated up to eightfold by sulfhydryl reagents was discovered in rat liver microsomes. This enzyme was subsequently purified to apparent homogeneity in 40 % yield using column cromatography. LÄS MER
3. Sorting and Import of Plant Mitochondrial Precursor Proteins
Sammanfattning : The mitochondrial genome encodes only a handful of the mitochondrial proteins; hundreds of mitochondrial proteins are encoded in the nucleus, synthesised on cytosolic polyribosomes and subsequently imported into the organelle. Most of the proteins targeted to the mitochondria are synthesised as precursors with an N-terminal extension called presequence or targeting sequence. LÄS MER
4. Microsomal glutathione transferase : Species characteristics, catalysis and topology
Sammanfattning : The membrane-bound detoxication enzyme microsomal glutathione transferase is localized predominantly in the liver of mammalian species, where it is present in high concentrations in the endoplasmic reticulum and the outer mitochondrial membrane. The enzyme conjugates a broad range of hydrophobic xenobiotics bearing reactive electrophilic centers to glutathione, which facilitates deactivation and excretion. LÄS MER
5. Glutathione reductase from erythrocytes : purification, characterization and identification of functional histidine and arginine residues of the enzyme
Sammanfattning : This investigation aims at the development of a rapid, simple purification procedure for glutathione reductase and at an exploration of the chemical groups of the enzyme essential for activity.Glutathione reductase from porcine erythrocytes was purified to homogeneity by the use of affinity chromatography on 2’,5’ ADP-Sepharose 4B. LÄS MER
