Sökning: "Glycosylated analysis"

Visar resultat 1 - 5 av 39 avhandlingar innehållade orden Glycosylated analysis.

1. 1. Glypican-1: Structural and functional analysis of the N-glycosylated human protein

   Författare :Wael Awad Abdelhady; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteoglycans; glypicans; glypican-1; heparan sulphate; N-glycosylation; exostosin-proteins; X-ray crystallography; crystal dehydration; diffraction anisotropy; Small angle X-ray scattering SAXS9; protein spectroscopy;

   Sammanfattning : Glypicans are multifunctional cell surface heparan sulphate proteoglycans co-regulating numerous signalling pathways, and are thereby involved in the control of cellular division, differentiation, and morphogenesis. The heparan sulphate (HS) chains are responsible for many of those biological functions; nevertheless recent studies suggest functional roles for the glypican core proteins in mediating the signalling of various growth factors. LÄS MER

3. 2. Analysis of noncovalent and covalent protein-ligand complexes by electrospray ionisation mass spectrometry

   Författare :Gustav Sundqvist; Harry Brumer; Per Andrén; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; noncovalent complex; droplet fission; nano-electrospray ionisation; mass spectrometry; Analytical chemistry; Analytisk kemi;

   Sammanfattning : In this thesis, the application of electrospray ionisation mass spectrometry (ESI-MS) to the analysis of intact proteins is demonstrated. In papers I and II, the use of ESI-MS for the analysis of noncovalent protein-ligand complexes were discussed. LÄS MER

4. 3. Analysis of sterol metabolism in the pathogenic oomycetes Saprolegnia parasitica and Phytophthora infestans

   Författare :Paul Dahlin; Vincent Bulone; Lisbeth Jonsson; Katharina Pawlowski; Lauren Mckee; Sophia Ekengren; Folke Sitbon; Steven Kelly; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Plant Physiology; växtfysiologi;

   Sammanfattning : The primary objective of this thesis was to investigate the sterol metabolism of two pathogenic oomycetes, specifically the processes of sterol synthesis and sterol acquisition in the fish pathogen Saprolegnia parasitica (Saprolegniales) and the plant pathogen Phytophthora infestans (Peronosporales). Furthermore, the effects of steroidal glycoalkaloids from Solanaceous plants, on P. LÄS MER

5. 4. Symbiotic and pathogenic factors in plant-microbe interaction: Structural basis of C-glycoside metabolism and lipoprotein transport in bacteria

   Författare :Valentina Furlanetto; Christina Divne; Mats Sandgren; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Plant-Bacteria Interaction; Symbiotic Communication; C-Glycosylated Compounds; Enzymatic Deglycosylation; Pathogenic Interactions; Lipoprotein Localization; Lol System; Biotechnology; Bioteknologi;

   Sammanfattning : The communication between plants and bacteria involves a complex chemical signaling network that mediates responses to various biotic and abiotic stresses, as well as establishing symbiotic relationships between different organisms.The first part of the thesis focuses on a mechanism for symbiotic communication between plants and bacteria and more specifically on how C-glycosylated aromatic polyketide compounds produced by plants can be used as a mechanism for plants to communicate with beneficial bacteria. LÄS MER

7. 5. Improving the biocatalyst. Engineering of fungal oxidoreductases and thin-film electrodes for improvement of membraneless biofuel cells and carbohydrate biosensors

   Författare :Roberto Ortiz; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Cellobiose dehydrogenase; pyranose dehydrogenase; biosensor; enzymatic biofuel cell; carbon nanotubes; gold nanoparticles; direct electron transfer; flow-injection analysis; diazonium salts; self-assembled monolayers;

   Sammanfattning : This thesis presents investigations for the improvement of electrical contact and catalytical properties of two fungal redox enzymes, cellobiose dehydrogenase (CDH) and pyranose dehydrogenase (PDH), with electrodes and the use of the prepared electrodes as carbohydrate biosensors and in enzymatic biofuel cells. The performance of enzyme modified electrodes can be improved in two ways, by modifying the bioelement or by modifying the electrode. LÄS MER