Sökning: "Glycosylated analysis"

Visar resultat 1 - 5 av 38 avhandlingar innehållade orden Glycosylated analysis.

  1. 1. Glypican-1: Structural and functional analysis of the N-glycosylated human protein

    Författare :Wael Awad Abdelhady; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteoglycans; glypicans; glypican-1; heparan sulphate; N-glycosylation; exostosin-proteins; X-ray crystallography; crystal dehydration; diffraction anisotropy; Small angle X-ray scattering SAXS9; protein spectroscopy;

    Sammanfattning : Glypicans are multifunctional cell surface heparan sulphate proteoglycans co-regulating numerous signalling pathways, and are thereby involved in the control of cellular division, differentiation, and morphogenesis. The heparan sulphate (HS) chains are responsible for many of those biological functions; nevertheless recent studies suggest functional roles for the glypican core proteins in mediating the signalling of various growth factors. LÄS MER

  2. 2. Analysis of noncovalent and covalent protein-ligand complexes by electrospray ionisation mass spectrometry

    Författare :Gustav Sundqvist; Harry Brumer; Per Andrén; KTH; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; noncovalent complex; droplet fission; nano-electrospray ionisation; mass spectrometry; Analytical chemistry; Analytisk kemi;

    Sammanfattning : In this thesis, the application of electrospray ionisation mass spectrometry (ESI-MS) to the analysis of intact proteins is demonstrated. In papers I and II, the use of ESI-MS for the analysis of noncovalent protein-ligand complexes were discussed. LÄS MER

  3. 3. Analysis of sterol metabolism in the pathogenic oomycetes Saprolegnia parasitica and Phytophthora infestans

    Författare :Paul Dahlin; Vincent Bulone; Lisbeth Jonsson; Katharina Pawlowski; Lauren Mckee; Sophia Ekengren; Folke Sitbon; Steven Kelly; Stockholms universitet; []

    Sammanfattning : The primary objective of this thesis was to investigate the sterol metabolism of two pathogenic oomycetes, specifically the processes of sterol synthesis and sterol acquisition in the fish pathogen Saprolegnia parasitica (Saprolegniales) and the plant pathogen Phytophthora infestans (Peronosporales). Furthermore, the effects of steroidal glycoalkaloids from Solanaceous plants, on P. LÄS MER

  4. 4. Improving the biocatalyst. Engineering of fungal oxidoreductases and thin-film electrodes for improvement of membraneless biofuel cells and carbohydrate biosensors

    Författare :Roberto Ortiz; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Cellobiose dehydrogenase; pyranose dehydrogenase; biosensor; enzymatic biofuel cell; carbon nanotubes; gold nanoparticles; direct electron transfer; flow-injection analysis; diazonium salts; self-assembled monolayers;

    Sammanfattning : This thesis presents investigations for the improvement of electrical contact and catalytical properties of two fungal redox enzymes, cellobiose dehydrogenase (CDH) and pyranose dehydrogenase (PDH), with electrodes and the use of the prepared electrodes as carbohydrate biosensors and in enzymatic biofuel cells. The performance of enzyme modified electrodes can be improved in two ways, by modifying the bioelement or by modifying the electrode. LÄS MER

  5. 5. Early diagnosis of diabetic retinopathy by mass examinations in primary health care

    Författare :Lars B Bäcklund; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :amaurosis - amine oxidase copper-containing - benzylamine - blindness - diabetes mellitus complications - diabetic retinopathy - Estonia - HbA1c - he services - services research - hemoglobin A; glycosylated - incidence - mass screening - methylamine - photo graphy - physicians; family - prevalence - population - primary health care - prospective studies - registries - rehabilitation centers - semicarbazide-sensitive amine oxidase SSAO -Sweden - vascular adhesion protein- I - vision screening - vision; subnormal - visual acuity;

    Sammanfattning : The general objective of this thesis was to describe a system for fundus photography for early diagnosis of diabetic retinopathy, performed by mobile teams visiting primary health care centres in Stockholm County. Large-scale implementation showed this method to be feasible, acceptable, and popular. LÄS MER