Sökning: "Affibody"

Visar resultat 36 - 40 av 90 avhandlingar innehållade ordet Affibody.

  1. 36. Staphylococcal surface display for protein engineering and characterization

    Författare :John Löfblom; Stefan Ståhl; Henrik Wernérus; K. Dane Wittrup; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; affibody; combinatorial library; epitope mapping; Gram-positive bacteria; protein engineering; staphylococcal surface display; Molecular biology; Molekylärbiologi;

    Sammanfattning : Even though our understanding of mechanisms such as protein folding and molecular recognition is relatively poor, antibodies and alternative affinity proteins with entirely novel functions are today generated in a routine manner. The reason for this success is an engineering approach generally known as directed evolution. LÄS MER

  3. 37. Production and delivery of recombinant subunit vaccines

    Författare :Christin Andersson; KTH; []
    Nyckelord :Affibody; Affinity chromatography; Affinity purification; DNA immunization; Expression plasmid; Fusion protein; Hydrophobic tag; Iscoms; Lipid tagging; Malaria; Mammalian cell expression; Recombinant immunogen; Respiratory Syncytial Virus; Semliki Fo;

    Sammanfattning : Recombinant strategies are today dominating in thedevelopment of modern subunit vaccines. This thesis describesstrategies for the production and recovery of protein subunitimmunogens, and how genetic design of the expression vectorscan be used to adapt the immunogens for incorporation intoadjuvant systems. LÄS MER

  4. 38. Engineering of staphylococcal surfaces for biotechnological applications

    Författare :Henrik Wernérus; KTH; []
    Nyckelord :affibody; albumin binding protein; Bacterial surface display; cell immobilisation; bioremediation; combinatorial protein engineering; flow cytometry; Gram-positive; metal-binding; staphylococcal protein A; Staphylococcus carnosus; Staphylococcus xylo;

    Sammanfattning : The engineering of bacterial surfaces has in recent yearsattracted a lot of attention with applications in manydifferent areas of bioscience. Here we describe the use of twodifferent surface display systems for the gram-positivebacteria Staphylococcus carnosus and Staphylococcus xylosus invarious biotechnological applications. LÄS MER

  5. 39. Functional proteomics : Generation and analysis of cDNA-encoded proteins

    Författare :Susanne Gräslund; KTH; []
    Nyckelord :functional genomics; functional proteomics; gene characterization; E. coli expression; affinity purification; immunolocalization; mouse testis; spermatogenesis; rabbit antibodies; affibody; tektin; Anaphase-promoting complex or cyclosome.;

    Sammanfattning : .... LÄS MER

  7. 40. Engineering of Affibody molecules targeting the Alzheimer’s-related amyloid β peptide

    Författare :Hanna Lindberg; Stefan Ståhl; John Löfblom; Caroline Ekblad; Sara Snogerup Linse; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Affibody molecules; Alzheimer’s disease; AD; amyloid beta; Aß; combinatorial protein engineering; staphylococcal surface display; Bioteknologi; Biotechnology;

    Sammanfattning : .... LÄS MER