Sökning: "microbial cell factories"

Visar resultat 1 - 5 av 25 avhandlingar innehållade orden microbial cell factories.

  1. 1. Next generation tools for microbial cell factories

    Författare :Alister James Cumming; Daniel Daley; Neil Dixon; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Microbial cell factories; Recombinant protein production; Bacteria; Plasmid; Genetic sensor; Inducible promoter; Genetic cassette; biokemi; Biochemistry;

    Sammanfattning : The bacterium E. coli is a popular choice for expressing recombinant proteins. It is easy to culture and many molecular tools for are available for directing protein production. When producing recombinant proteins, high yields and good quality soluble products are desirable. LÄS MER

  2. 2. Droplet microfluidics for screening and sorting of microbial cell factories

    Författare :Sara Björk; Helene Svahn Andersson; Håkan Jönsson; Christoph Merten; KTH; []
    Nyckelord :Droplet microfluidics; Cell factories; High-throughput screening; Cell culture; Bioteknologi; Biotechnology;

    Sammanfattning : Cell factories are cells that have been engineered to produce a compound of interest, ranging from biopharmaceuticals to biofuels. With advances in metabolic engineering, the number of cell factory variants to evaluate has increased dramatically, necessitating screening methods with increased throughput. LÄS MER

  3. 3. Engineering microbial cell factories for protein production

    Författare :Kiavash Mirzadeh; Daniel Daley; Grzegorz Kudla; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Protein production; expression vector; recombinant DNA; Translation initiation region; Escherichia coli; mRNA secondary structure; Synthetic evolution; Biochemistry; biokemi;

    Sammanfattning : Proteins are often produced using microbial cell factories for academic or industrial purposes. Protein production is however not an open-and-shut procedure. Production yields often vary in an unpredictable and context dependent manner, limiting the rational design of a straightforward production experiment. LÄS MER

  4. 4. Towards membrane engineering as a tool in cell factory design: A case study on acetic acid tolerance in Saccharomyces cerevisiae

    Författare :Lina Lindahl; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; butanol; inhibitors; lipidomics; Key words: Lignocellulose; Zygosaccharomyces bailii; molecular dynamics simulations; membrane permeability; carbon-14 uptake; ethanol; robustness;

    Sammanfattning : The sustainable production of fuels, chemicals, and materials using renewable resources is a necessity if we are to reduce our ecological footprint and the rate of climate change. Lignocellulosic biomass, the major constituent of plant cell walls, is a renewable raw material with great potential due to its high abundance. LÄS MER

  5. 5. Understanding and improving microbial cell factories through Large Scale Data-approaches

    Författare :Daniel Brink; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Microbiology; lignocellulose; lignin; D-xylose; Saccharomyces cerevisiae; Pseudomonas putida; bioinformatics; genome assembly; flow cytometry; metabolic engineering; sugar sensing signalling;

    Sammanfattning : Since the advent of high-throughput genome sequencing methods in the mid-2000s, molecular biology has rapidly transitioned towards data-intensive science. Recent technological developments have increased the accessibility of omics experiments by decreasing the cost, while the concurrent design of new algorithms have improved the computational work-flow needed to analyse the large datasets generated. LÄS MER