Sökning: "mediated amperometry"

Hittade 5 avhandlingar innehållade orden mediated amperometry.

  1. 1. PROBING NAD(P)H AVAILABILITY IN LIVING CELLS USING MINIATURIZED ELECTROCHEMICAL SYSTEMS

    Författare :Natalie Kostesha; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; redox; NAD P H; living cells; S. cerevisiae; mediated amperometry; microelectrode; electrochemistry;

    Sammanfattning : The work presented in this thesis demonstrates the application of electrochemical methods for probing the dynamics of cellular metabolism in prokaryotic and eukaryotic cells. The main part of the work has been done using genetically modified strains of yeast. Employing baker’s yeast, S. LÄS MER

  3. 2. Monitoring of Cellular Dynamics with Focus on Electrochemical Detection Techniques

    Författare :Arto Heiskanen; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Microfluidic system; Microchip; PC12; Saccharomyces cerevisiae; Electrochemical impedance spectroscopy; Cell adhesion; Amperometry; Exocytosis; Redox environment; Cell-based assay;

    Sammanfattning : This thesis presents work in two main areas of focus, the development of electroanalytical techniques to monitor the dynamics of cellular processes as well as design and fabrication of microelectrode chips for electrochemical detection as well as a prototype microfluidic system to be used for cell analysis and culturing. The electrochemically monitored cellular parameters were the cellular redox environment (CRE), cell adhesion and exocytosis. LÄS MER

  4. 3. 2nd and 3rd generation electrodes for biosensors and biofuel cells

    Författare :MNadeem Zafar; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; WrbA; pyranose dehydrogenase; cellobiose dehydrogenase; glucose dehydrogenase; direct electron transfer; Os-polymers; mediated electron transfer; single-walled carbon nanotubes; amperometric biosensor; biofuel cell; screen-printed electrodes; flow injection analysis; cascade reaction; bioanode;

    Sammanfattning : The catalytic and electrochemical properties of tryptophan repressor-binding protein (WrbA), pyranose dehydrogenase (PDH), cellobiose dehydrogenase (CDH) and glucose dehydrogenase (GDH) on carbon electrodes were investigated using cyclic voltammetry and flow injection amperometry to construct biosensors and biofuel cell (BFC) anodes. WrbA has a catalytic FMN-containing domain whereas both PDH and GDH have a catalytic FAD-containing domains. LÄS MER

  5. 4. Engineering of thermometric biosensors and characterisation of redox enzymes for improved performance of biosensors/biofuel cells

    Författare :Maria Yakovleva; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Flow injection analysis; thermometric calorimetric biosensor; enzyme thermistor; immobilised enzyme reactor; amperometric biosensor; enzymatic biofuel cell; bioanode; pyranose dehydrogenase; cellobiose dehydrogenase; direct electron transfer; mediated electron transfer; matrix-assisted laser desorption ionization; glycosite mapping;

    Sammanfattning : The present work is mainly focused on the development of enzyme-based bioanalytical devices, which can further be applied as analytical tools for industrial, medical and environmental applications. The first part of the work deals with the practical applications of thermometric biosensors for determination of various sugars (fructose and lactose) in a real samples. LÄS MER

  7. 5. Heterogeneous electron transfer studies with ligninolytic redox enzymes and living bacteria. Applications in biosensors and biofuel cells

    Författare :Vasile Coman; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; cellobiose dehydrogenase; direct electron transfer; mediated electron transfer; self-assembled monolayer; flow injection analysis; amperometric biosensor; E. coli; succinate:quinone reductase; biofuel cell; B. subtilis; whole-cell biosensor;

    Sammanfattning : The catalytic properties and the inter-domain electron transfer of cellobiose dehydrogenase (CDH) from the ascomycete fungus Myriococcum thermophilum adsorbed on graphite and thiol (SAM) modified gold electrodes were investigated using cyclic voltammetry, flow injection amperometry and UV-Vis spectroelectrochemistry. The fabrication and characterisation of a noncompartmentalised, mediator and cofactor free glucose–oxygen biofuel cell well-operating in glucose-containing buffers and human blood serum was performed. LÄS MER