Sökning: "whole-cell biosensor"

Hittade 5 avhandlingar innehållade orden whole-cell biosensor.

  1. 1. Heterogeneous electron transfer studies with ligninolytic redox enzymes and living bacteria. Applications in biosensors and biofuel cells

    Författare :Vasile Coman; Centrum för analys och syntes; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; cellobiose dehydrogenase; direct electron transfer; mediated electron transfer; self-assembled monolayer; flow injection analysis; amperometric biosensor; E. coli; succinate:quinone reductase; biofuel cell; B. subtilis; whole-cell biosensor;

    Sammanfattning : The catalytic properties and the inter-domain electron transfer of cellobiose dehydrogenase (CDH) from the ascomycete fungus Myriococcum thermophilum adsorbed on graphite and thiol (SAM) modified gold electrodes were investigated using cyclic voltammetry, flow injection amperometry and UV-Vis spectroelectrochemistry. The fabrication and characterisation of a noncompartmentalised, mediator and cofactor free glucose–oxygen biofuel cell well-operating in glucose-containing buffers and human blood serum was performed. LÄS MER

  3. 2. An NADH-Coupled Biosensor for Engineering Redox Metabolism in Saccharomyces cerevisiae

    Författare :Jan Knudsen; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Yeast; redox balance; glycerol whole-cell biocatalysis; single-cell monitoring; FACS;

    Sammanfattning : Baker’s yeast, Saccharomyces cerevisiae’s potential in industrial biotechnology for producing valuable products e.g. biofuels, bulk chemicals, bio-flavours and pharmaceuticals is established. LÄS MER

  4. 3. Biosynthesis of capsaicinoids by recombinant Saccharomyces cerevisiae

    Författare :Nina Muratovska; Teknisk mikrobiologi; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; capsaicinoids; nonivamide; baker’s yeast; Capsicum; Saccharomyces cerevisiae; Pseudomonas putida; lignin-derived substrates; vanillin aminotransaminase; reductive amination; vanillylamine; N-Acyltransferase; CoA-ligase; amide; whole-cell bioconversion; TRPV1 receptor; ion channel; calcium dye Fluo-4; flow cytometry;

    Sammanfattning : Throughout history, people have used products from different microorganisms and plants for many medicinal and nutritional applications. Chilli peppers have been used for spicing up food, but also for their pharmacological properties. LÄS MER

  5. 4. Adaptive Responses by Transcriptional Regulators to small molecules in Prokaryotes : Structural studies of two bacterial one-component signal transduction systems DntR and HpNikR

    Författare :Cyril Dian; Peter Brzezinski; Séan McSweeney; Elisabeth Sauer-Eriksson; Stockholms universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; transcription regulator; bacterial biosensor; LysR family; Inducer-binding; nickel-sensing; NikR; signal transduction system; Helicobacter pylori; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : Prokaryotes are continually exposed to variations in their environment. Survival in unstable milieu requires a wide range of transcriptional regulators (TRs) that respond to specific environmental and cellular signals by modulating gene expression and provide an appropriate physiological response to external stimuli. LÄS MER

  7. 5. QCM-based sensing using biological and biomimetic interfaces

    Författare :Louise Elmlund; Ian Nicholls; Teodor Aastrup; Jesper Wiklander; Kristina Nilsson-Ekdahl; Håkan Andersson; Susanne Wikman; Alessandra Maria Bossi; Linnéuniversitetet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Quartz crystal microbalance; molecularly imprinted polymers; phage display; interactions; artificial receptors; recognition; selectivity; Kemi; Chemistry;

    Sammanfattning : The objective of this thesis was to explore novel approaches for studying molecular recognition at biological and biomimetic surfaces using the quartz crystal microbalance (QCM) biosensor technique. The first two papers focused on the synthesis and study of biotin selective polymer films prepared using the molecularly imprinted polymer (MIP) technique. LÄS MER