Sökning: "ion exchange chromatography"

Visar resultat 16 - 20 av 56 avhandlingar innehållade orden ion exchange chromatography.

  1. 16. Purification Processes for Complex Biomacromolecules

    Författare :Hans Blom; Helena Grennberg; Mats Lundgren; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Chromatography; enzymes; mucins; fractionation; plasmid DNA; analysis; clarification; process design; Chemistry with specialization in Surface Biotechnology; Kemi med inriktning mot ytbioteknik;

    Sammanfattning : This thesis details various techniques and considerations for the purification of complex biomacromolecules. Initially an α-mannosidase from babaco fruit was purified using anion exchange-, lectin affinity- and size exclusion chromatography. LÄS MER

  3. 17. Strategies for facilitated protein recovery after recombinant production in Escherichia coli

    Författare :My Hedhammar; Sophia Hober; Alois Jungbauer; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ion-exchange chromatography; protein A; Z; Zbasic; Zacid; fusion protein; proteolytic cleavage; immobilised protease; flow cytometry; inclusion bodies; solid-phase refolding; Molecular biology; Molekylärbiologi;

    Sammanfattning : The successful genomic era has resulted in a great demand for efficient production and purification of proteins. The main objective of the work described in this thesis was to develop methods to facilitate recovery of target proteins after recombinant production in Escherichia coli. LÄS MER

  4. 18. Polymer Displacement and Supermacroporous Cryogels - Two New Tools in Chromatographic Separation of Biomolecules and Bioparticles

    Författare :Pär Arvidsson; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; biopolymers; Polymerteknik; Biochemical technology; Polymer technology; IMAC; dye-affinity; affinity chromatography; viable cells; cell chromatography; non-clarified cell homogenate; cryogel; Polymer displacement; supermacroporous; Biokemisk teknik;

    Sammanfattning : Production of protein is often accompanied with difficulties due to the large amounts of different biological material resulting from fermentation. This makes purification necessary and affinity chromatography is usually involved as being biospecific, although suffering from limitations as low recovery due to ineffective elution and inability to handle particulate materials. LÄS MER

  5. 19. Composite Cryogels: Stationary Phase for Separation of Complex Media

    Författare :Solmaz Hajizadeh; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Composite cryogel; Complex media; Purification; Chromatography; Environmental application; Molecularly imprinted polymer;

    Sammanfattning : Demands for efficient, economical and fast separation and purification methods for various target molecules have led to the development of new techniques and materials for bioseparation processes. Separation can be carried out in both batch and chromatographic modes. LÄS MER

  7. 20. Lignin Model Compounds: Synthesis, Stereochemistry and Acidolysis

    Författare :Shiming Li; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; boric acid complexes; Arylglycerol b-aryl ethers; stereochemistry; 3-Diaryl-2-propen-1-ols; 1; Phenylcoumarans; lignin model compounds; acidolysis; ion-exchange chromatography; 3-propanediols; 2-Diaryl-1; 2; X-ray crystallography;

    Sammanfattning : Lignin model compounds representative of structural elements in lignin of the b-1, b-5, b-O-4, cinnamaldehyde and cinnamyl alcohol types were synthesized. erythro-Forms of b-1 models of the 1,2-diaryl-1,3-propanediol type were prepared by BF3-catalyzed rearrangement of chalcone epoxides and subsequent reduction of the rearrangement products with NaBH4. LÄS MER