Sökning: "enzymatic mechanism"

Visar resultat 16 - 20 av 162 avhandlingar innehållade orden enzymatic mechanism.

  1. 16. Quantum chemical modeling of enzymatic reactions : applications to the tautomerase superfamily

    Författare :Robin Sevastik; Fahmi Himo; Margaretha Blomberg; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Bioengineering; Bioteknik;

    Sammanfattning : In this thesis, quantum chemical methods are used to investigate enzymatic reaction mechanisms. The Density functional theory, in particular the hybrid B3LYP functional, is used to model two enzymes belonging to the tautomerase superfamily; 4-Oxalocrotonate Tautomerase (4-OT) and cis-Chloroacrylic Acid Dehalogenase (cis-CAAD). LÄS MER

  3. 17. The kinetics of cellulose enzymatic hydrolysis : Implications of the synergism between enzymes

    Författare :Priit Väljamäe; Jan-Christer Janson; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Acetobacter; Cellobiohydrolase; Cellobiose; Cellulase; Cellulose; Diffusion; Endoglucanase; Hydrolysis; Inhibition; Kinetics; Model; Product; Substrate; Surface; Synergism; Trichoderma reesei; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

    Sammanfattning : The hydrolysis kinetics of bacterial cellulose and its derivatives by Trichoderma reesei cellulases was studied. The cellulose surface erosion model was introduced to explain the gradual and strong retardation of the rate of enzymatic hydrolysis of cellulose. LÄS MER

  4. 18. Quantum Chemical Studies of Enzymatic Reaction Mechanisms : Investigations of Cytosine Deaminase and ω-Transaminase

    Författare :Bianca Manta; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : In this thesis, density functional theory is used to study the reaction mechanisms of two dierent enzymes. Quantum chemical cluster models of the active sites were designed using available crystal structures. LÄS MER

  5. 19. Quantum Chemical Modeling of Asymmetric Enzymatic Reactions : Applications to Limonene Epoxide Hydrolase and Arylmalonate Decarboxylase

    Författare :Maria E.S. Lind; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : In this thesis, density functional theory has been employed to study the reactionmechanisms of two enzymes with possible applications in asymmetric biocatalysis.To reproduce and rationalize the stereoselectivity of the enzymes, quite large cluster models that account for the chiral environment of the active site have been used. LÄS MER

  7. 20. Crystallographic studies of transaldolase : implications for the enzymatic mechanism and the evolution of class I aldolases

    Författare :Jia Jia; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :transaldolase; aldolase; Schiff-base intermediate; protein crystallography; site directed mutagenesis; catalytic mechanism; circular permutation.;

    Sammanfattning : Transaldolase catalyzes the reversible transfer of a dihydroxyacetone moiety from a ketose donor to an aldose acceptor by forming a Schiff-base intermediate between a Iysine residue and dihydroxyacetone. It belongs to the class I aldolase family. LÄS MER