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Visar resultat 1 - 5 av 6 avhandlingar som matchar ovanstående sökkriterier.

1. 1. Redesign of Alpha Class Glutathione Transferases to Study Their Catalytic Properties

   Författare :Lisa O Nilsson; Ralf Morgenstern; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; glutathione transferase; heterologous expression; heterodimer; hydroxyalkenal; protein redesign; sequential design; dimer-interface interactions; enzyme kinetics; glutathione; dynamic C-terminus; viscosity dependence; Biokemi; Biochemistry; Biokemi; biokemi; Biochemistry;

   Sammanfattning : A number of active site mutants of human Alpha class glutathione transferase A1-1 (hGST A1-1) were made and characterized to determine the structural determinants for alkenal activity. The choice of mutations was based on primary structure alignments of hGST A1-1 and the Alpha class enzyme with the highest alkenal activity, hGST A4-4, from three different species and crystal structure comparisons between the human enzymes. LÄS MER

3. 2. Protein-protein interactions in model systems : design, control of catalytic activity and biosensor applications

   Författare :Johan Rydberg; Lars Baltzer; Joel Schneider; Linköpings universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Chemistry; Protein interactions; design; catalysis; biosensors; hybride materials; nanoparticles; Kemi; Chemistry; Kemi;

   Sammanfattning : This thesis describes the design of polypeptides, unordered in the monomeric state but capable of folding into helix-loop-helix motifs and dimerise to form four-helix bundles. The goal of the design was to encode them with the capacity to form dimers highly selectively and the ability to carry out molecular functions in the folded state but not in the unordered state, and thus to establish a molecular link between recognition and function. LÄS MER

4. 3. Structural studies of drug targets and a drug metabolizing enzyme

   Författare :Daniela Cederfelt; U. Helena Danielson; Doreen Dobritzsch; Inari Kursula; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biophysics; Protein structure; X-ray crystallography; Cryogenic electron microscopy; Enzymology; Drug discovery; Pyrimidine degradation; Biokemi; Biochemistry;

   Sammanfattning : The work presented in this thesis describes how structural information about a protein can be acquired, and how it can be used to answer scientific questions about proteins’ function, their dynamic behaviour and their interactions with other proteins or ligands.The catalytic function of the pyrimidine-degrading, drug metabolizing enzyme β-ureidopropionase (βUP) is dependent on the shift between oligomeric states. LÄS MER

5. 4. Computational Studies of UV-B Induced Signalling Pathways in Plants

   Författare :Min Wu; Göteborgs universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Computational Chemistry;

   Sammanfattning : Abstract This thesis presents theoretical studies of UV-B induced signaling pathways in plants using molecular dynamic methods combined with (TD)-DFT calculations. Ultraviolet B (UV-B) radiation is a component of sunlight covering wavelengths 280-300nm. LÄS MER

7. 5. Crystallographic studies of gluconate kinase

   Författare :Louise Kraft; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : The first steps of gluconate metabolism in Escherichia coli are the transport of gluconate into the cell by a gluconate transport system, followed by phosphorylation to gluconate-6- phosphate by gluconate kinase. There exist two such different pathways, Grill and GntIl, respectively. LÄS MER