Sökning: "cellular crowding"

Visar resultat 1 - 5 av 11 avhandlingar innehållade orden cellular crowding.

  1. 1. Navigating the Cellular Crowd : Physicochemical Properties of Protein Surfaces as Evolved Interaction Guides

    Författare :Eloy Vallina Estrada; Mikael Oliveberg; Erik Lindahl; Gideon Schreiber; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein interactions; inteins; physicochemical properties; protein surfaces; evolution; cellular crowding; electrostatics; Biophysics; biofysik;

    Sammanfattning : The cellular interior is characterised by high concentrations of macromolecules. Compared with dilute conditions, the crowd modifies proteins' ability to fold, diffuse and, ultimately, carry out their biological functions. Cellular fitness depends on ensuring an adequate balance between interactivity and diffusivity. LÄS MER

  3. 2. Effects of Macromolecular Crowding on Protein Folding : - in-vitro equilibrium and kinetic studies on selected model systems

    Författare :Alexander Christiansen; Pernilla Wittung-Stafshede; Thomas Kiefhaber; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Protein Folding; macromolecular crowding; excluded volume; spectroscopy; stopped-flow; biokemi; Biochemistry;

    Sammanfattning : Protein folding is the process during which an extended and unstructured polypeptide converts to its compact folded structure that is most often the functional state. The process has been characterized extensively in dilute buffer in-vitro during the last decades but the actual biological place for this process is the inside of living cells. LÄS MER

  4. 3. Analysis of Binding Events and Diffusion in Living Cells

    Författare :Markus Elsner; Göteborgs universitet; []
    Nyckelord :COPI; Golgi; Diffusion; Glycosyltransferases; Sorting; Molecular; Crowding;

    Sammanfattning : It is well known that diffusion is the main mode of transport in living cells, but the consequences of diffusion in a complex cellular environment are not generally appreciated. In this thesis, we have investigated several aspects of how diffusion properties influence the observability of cellular binding kinetics and how they can be used to obtain information about the environment of proteins and other molecules. LÄS MER

  5. 4. Peptide folding and local unfolding of SOD1 in the presence of interacting macromolecular crowders - a Monte Carlo approach

    Författare :Anna Bille; Beräkningsbiologi och biologisk fysik - Genomgår omorganisation; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Monte Carlo simulations; protein folding; SOD1; crowding; Fysicumarkivet A:2016:Bille;

    Sammanfattning : A protein chain often folds into a functional, specific three dimensional structure. Failurein this process can in some cases lead to disease, e.g. the misfolding disease ALS. LÄS MER

  7. 5. Effect of Macromolecular Crowding on Diffusive Processes

    Författare :Zahedeh Bashardanesh; David van der Spoel; Per Lötstedt; Lars Schäfer; Uppsala universitet; []
    Nyckelord :;

    Sammanfattning : Macromolecular crowding are innate to cellular environment. Understanding their effect on cellular components and processes is essential. This is often neglected in dilute experimental setup both in vitro and in silico. LÄS MER