Sökning: "cell factories"

Visar resultat 21 - 25 av 52 avhandlingar innehållade orden cell factories.

1. 21. Not just protein factories : role of ribosome biogenesis as an architect of epithelial-mesenchymal transition and breast cancer progression

   Författare :Varsha Prakash; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : RNA polymerase I (Pol I)-mediated transcription of ribosomal DNA (rDNA) is considered to be the rate-limiting step in ribosome biogenesis and is a well-known hallmark of cell growth and proliferation. The process of synthesizing new ribosomes is executed by the coordination of multiple complex processes in the nucleolus. LÄS MER

3. 22. Robustness quantification in yeast: A methodology to study phenotypic, evolutionary, and genomic aspects of microbial robustness

   Författare :Cecilia Trivellin; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; bioproduction; Saccharomyces cerevisiae; adaptive laboratory evolution; High-throughput; fluctuating conditions; perturbations;

   Sammanfattning : Bioprocesses contribute to the shift towards a more sustainable economy. In bioprocesses, valuable chemicals can be generated from renewable resources while, at the same time, reducing carbon emissions. A major hurdle in bringing bio-based products to market is the time and cost involved in designing efficient cell factories. LÄS MER

4. 23. Engineering the Secretory Pathway for Recombinant Protein Secretion in Saccharomyces cerevisiae

   Författare :Jichen Bao; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; endoplasmic reticulum; oxidative stress; GLO3; protein secretion; transcriptomics; drug screening; Saccharomyces cerevisiae; SEC16;

   Sammanfattning : In the past few decades there has been an increasing demand of biopharmaceutical proteins in the market. Several types of cell factories are applied to produce different pharmaceutical proteins. LÄS MER

5. 24. Systems Biology of Protein Secretion in Human Cells: Multi-omics Analysis and Modeling of the Protein Secretion Process in Human Cells and its Application

   Författare :Rasool Saghaleyni; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Erythropoietin; cancer protein secretory pathway; protein secretion modeling; HEK293; protein secretion; Integrative omics analysis; genome-scale modeling;

   Sammanfattning : Since the emergence of modern biotechnology, the production of recombinant pharmaceutical proteins has been an expanding field with high demand from industry. Pharmaceutical proteins have constituted the majority of top-selling drugs in the pharma industry during recent years. LÄS MER

7. 25. Metabolic Engineering of Recombinant Protein Production by Saccharomyces cerevisiae

   Författare :Zihe Liu; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; recombinant protein production; genome sequencing; DNA microarray; insulin precursor; growth rate; UV mutation; yeast; anaerobic electron acceptor; secretory pathway; unfolded protein response; metabolic engineering; systems biology; α-amylase;

   Sammanfattning : The yeast Saccharomyces cerevisiae is a widely used cell factory for the production of fuels, chemicals, and it also provides a platform for the production of many heterologous proteins of medical or industrial interest. In this thesis, random and rational approaches, such as vector design, host engineering, fermentation analysis, UV Mutation, coupled with high-throughput systems biology techniques (including whole genomic sequencing, microarray analysis and flux analysis) and integrated analysis (Reporter feature technique), were employed to engineer cellular properties more effectively and purposefully to construct cell factories for protein production. LÄS MER