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1. 1. Maturation and Regulation of Cyanobacterial Hydrogenases

   Författare :Åsa Agervald; Peter Lindblad; Karin Stensjö; Ann Magnuson; Poul Erik Jensen; Uppsala universitet; []
   Nyckelord :Biohydrogen; Cyanobacteria; Nostoc sp. PCC 7120; Nostoc punctiforme PCC 73102; Hydrogenase; Maturation; Transcriptional regulation; CyAbrB; CalA; FeSOD;

   Sammanfattning : Accelerated global warming plus an increasing need for energy is an equation not easily solved, thus new forms of sustainable energy production are urgently requested. In this context hydrogen production based on a cyanobacterial system offers an environmentally friendly alternative for energy capture and conversion. LÄS MER

3. 2. Hydrogenases in the Cyanobacterium Nostoc sp. Strain PCC 73102

   Författare :Fredrik Oxelfelt; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Plant physiology; Växtfysiologi; Plant physiology; Växtfysiologi; fysiologisk botanik; Physiological Botany;

   Sammanfattning : Many cyanobacteria are capable of fixing atmospheric N2, a reaction that is also producing H2. This hydrogen is often metabolized by an enzyme called hydrogenase. The aim of the present work was to identify and characterize hydrogen metabolism/hydrogenases in the free-living N2-fixing cyanobacterium Nostoc sp.strain PCC 73102. LÄS MER

4. 3. Cyanobacterial Hydrogen Metabolism - Uptake Hydrogenase and Hydrogen Production by Nitrogenase in Filamentous Cyanobacteria

   Författare :Pia Lindberg; Peter Lindblad; Kornél Kovács; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Microbiology; cyanobacteria; biohydrogen; Nostoc; hydrogen production; hydrogen evolution; hydrogen metabolism; nitrogenase; hydrogenase; hydrogen; uptake hydrogenase; hupSL; hydrogen uptake mutant; uptake hydrogenase mutant; photobioreactor; hydrogenase accessory genes; hyp; transcription; RT-PCR; NtcA; mass spectrometry; heterocyst; Mikrobiologi; Microbiology; immunology; infectious diseases; Mikrobiologi; immunologi; infektionssjukdomar;

   Sammanfattning : Molecular hydrogen is a potential energy carrier for the future. Nitrogen-fixing cyanobacteria are a group of photosynthetic microorganisms with the inherent ability to produce molecular hydrogen via the enzyme complex nitrogenase. This hydrogen is not released, however, but is recaptured by the bacteria using an uptake hydrogenase. LÄS MER

5. 4. The Cyanobacterial Uptake Hydrogenase : Regulation, Maturation and Function

   Författare :Marie Holmqvist; Peter Lindblad; Karin Stensjö; Kornél Kovács; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biohydrogen; CalA; Cyanobacteria; Hydrogenase; Maturation; Nostoc punctiforme ATCC 29133; Nostoc sp. PCC 7120; Transcriptional regulation; Molecular biology; Molekylärbiologi; Molecular biology; Molekylärbiologi; Chemistry with specialization in Microbial Chemistry; Kemi med inriktning mot mikrobiell kemi;

   Sammanfattning : With accellerating global warming and pollution problems a change of energy regime is necessary. Solar energy offers a clean and unlimited energy source of enormous potential. Due to it’s intermittenet nature solar energy must be stored - ideally in the chemical bond of a carrier molecule. LÄS MER

7. 5. Development of robust cyanobacterial strains for biotechnological applications : Stress tolerance and cell-specific expression in heterocyst-forming cyanobacteria

   Författare :Xin Li; Karin Stensjö; Yagut Allahverdiyeva-Rinne; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Cyanobacteria; Dps protein; heterocysts; Nostoc; oxidative stress; cell specific promoter; ROS tolerance; synthetic biology tools transcriptional regulation.; Biokemi; Biochemistry;

   Sammanfattning : Synthetic biology tools can be used to exploit potential applications of cyanobacteria, promising photosynthetic hosts for production of fuels and chemicals. Specific genetic tools are needed for the development of robust cyanobacterial strains for bioengineering. LÄS MER