Sökning: "MALDI-TOF"

Visar resultat 1 - 5 av 62 avhandlingar innehållade ordet MALDI-TOF.

1. 1. Sample Handling Techniques in Biological and Environmental Applications Emphasizing Biomolecular Recognition and MALDI-TOF MS

   Författare :Patrik Önnerfjord; Reumatologi och molekylär skelettbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein identification; microchip IMER; microdispensing; MALDI-TOF MS; fluorescence polarization; flow immunoassay; pesticide immunoassays; phenol biosensor; restricted access media; SPE; Analytical chemistry; Analytisk kemi;

   Sammanfattning : The use of restricted access media and other stationary phases for clean-up and trace enrichment of environmental samples coupled on-line to liquid chromatography using diode array detection as well as thermospray MS is described. This on-line configuration was compared with commercially available ELISAs for the determination of triazines in complex matrices, showing superior precision and accuracy with the chromatographic method. LÄS MER

3. 2. Analysis of proteins related toautoimmune and inflammatorydiseases with focus on newenrichment methodologies and MALDI-TOF MS

   Författare :Yuye Zhou; Åsa Emmer; Margret Thorsteinsdottir; KTH; []
   Nyckelord :Protein; Glycopeptide; IgG; Osteopontin; Enrichment; Digestion; Separation; Microchip; MALDI-TOF MS; Kemi; Chemistry;

   Sammanfattning : Proteins have been widely studied in biological sample analysis due to the association with diseases. In the present project, method and technique development was carried out on the analysis of two proteins, immunoglobulin G (IgG) and osteopontin (OPN). LÄS MER

4. 3. Sampling and Selective Detection of Peptides and Proteins Emphazing Microdialysis,Bio-recognition Assays and MALDI-TOF MS

   Författare :Sven Kjellström; Centrum för analys och syntes; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; cyclodextrines; protein identification; m-dispenser; push-pull microdialysis; microdialysis sampling; MALDI-TOF MS; Interleukin-8; Flow immuno assays; Interleukin-10; Analytical chemistry; Analytisk kemi;

   Sammanfattning : The use of microdialysis sampling and flow immunochemical detection as well as MALDI-TOF MS for peptide and protein analysis is described. Sampling of peptides and smaller proteins was investigated with different membranes. LÄS MER

5. 4. Improved mass accuracy in MALDI-TOF-MS analysis

   Författare :Martin Kempka; Johan Roeraade; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; MALDI; mass spectrometry; mass accuracy; peak picking; ion formation; proteins; peptides; chip; sample handling; sensitivity.; Analytisk kemi; Analytical chemistry; Analytisk kemi;

   Sammanfattning : Mass spectrometry (MS) is an important tool in analytical chemistry today, particularly in the field of proteomics where identification of proteins is the central activity. The focus in this thesis has been to improve the mass accuracy of MS-analyses in order to improve the possibility for unambiguous identification of proteins. LÄS MER

7. 5. New Tools for Analysis of Wood and Pulp Components - Application of MALDI-TOF Mass Spectroscopy and Capillary Zone Electrophoresis

   Författare :Anna Jacobs; KTH; []
   Nyckelord :Capillary zone electrophoresis; carbohydrate composition; degree of acetylation; degree of polymerization; enzymatic hydrolysis; hardwood; hemicellulose; hexenuronic acid; lignin; MALDI-TOF mass spectroscopy; molar mass; molar mass distribution; olig;

   Sammanfattning : In the present thesis, capillary zone electrophoresis (CZE),matrix-assisted laser desorption/ionization time-of-flight massspectroscopy (MALDI-MS) and size-exclusion chromatography incombination with off-line MALDI-MS (SEC/MALDI-MS) have beenapplied successfully to the analysis of wood and pulpcomponents.The carbohydrate compositions of pulp and holocellulosesamples, as well as of isolated hemicellulose preparations,were determined employing enzymatic hydrolysis and subsequentanalysis of the saccharides released by CZE. LÄS MER